Cotton Rat IL-10 Antibody

Catalog #: MAB581 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB581
MAB581-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Cotton Rat IL-10 Antibody Summary

Species Reactivity

Cotton Rat

Specificity

Detects recombinant cotton rat IL-10 in direct ELISAs and Western blots. In Western blots, 50% cross-reactivity with recombinant rat IL‑10 and recombinant mouse IL-10 is observed and no cross-reactivity with recombinant canine IL-10, recombinant feline IL-10, recombinant human IL-10, recombinant porcine IL-10, recombinant viral IL-10, or recombinant mouse IL-22 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 155115

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant cotton rat IL‑10
Ser1-Ser160 (Cys149Tyr)
Accession # AAK94013

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Cotton Rat IL‑10 (Cys149Tyr) (Catalog # 581-CR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones that is dependent upon stimulation with antigen in the presence of antigen presenting cells (APC).

IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

References

Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF