Cotton Rat IL-4 Antibody

  
  • Species Reactivity
    Cotton Rat
  • Specificity
    Detects cotton rat IL‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat IL-4 is observed, 5% cross-reactivity with recombinant mouse IL-4 is observed, and less than 1% cross-reactivity with recombinant human IL-4 and recombinant porcine IL-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant cotton rat IL‑4
    Cys21-Phe147
    Accession # AAL18820
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Cotton Rat IL‑4 (Catalog # 584-R4)
  • Neutralization
    Measured by its ability to neutralize IL‑4-induced proliferation in the rat splenocytes. The Neutralization Dose (ND50) is typically 0.075-0.3 µg/mL in the presence of 10 ng/mL Recombinant Cotton Rat IL‑4 and 10 µg/mL PHA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑4 and Neutralization by Cotton Rat IL‑4 Antibody. Recombinant Cotton Rat IL‑4 (Catalog # 584-R4) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL‑4 (10 ng/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL‑4 Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF584). The ND50 is typically 0.075-0.3 µg/mL in the presence of PHA (10 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Cotton rat IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature cotton rat IL-4 shares 41%, 44%, 57%, and 68% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (5, 6). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (7‑10). IL-4 plays a dominant role in the development of allergic inflammation and asthma (9, 11).

  • References:
    1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
    2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
    3. Accession # AAL18820.
    4. Redfield, C. et al. (1991) Biochemistry 30:11029.
    5. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
    6. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
    7. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
    8. Corthay, A. (2006) Scand. J. Immunol. 64:93.
    9. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
    10. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
    11. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  • Long Name:
    Interleukin 4
  • Entrez Gene IDs:
    3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
  • Alternate Names:
    B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
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