Detects equine IL-1 beta /IL-1F2 in direct ELISAs. In direct ELISAs, 100% cross‑reactivity with recombinant canine, guinea pig, mouse, rat, and porcine IL‑1 beta is observed and 10‑50% cross‑reactivity with recombinant feline, human, rabbit, and rhesus IL‑1 beta is observed. Approximately 20% cross‑reactivity with recombinant mouse (rm) IL‑36 alpha and no cross‑reactivity with recombinant human (rh) IL‑36 alpha, rhIL‑36 gamma, rhIL‑1F10, rhIL‑36Ra, rmIL‑1Ra, rmIL‑18, or rmIL‑36 beta is observed.
Monoclonal Mouse IgG1 Clone # 608714
Protein A or G purified from hybridoma culture supernatant
IL‑1 beta /IL‑1F2 in Equine PBMCs.
IL‑1 beta /IL‑1F2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Equine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB33401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta shares 65%-75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.
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