Key Product Details

Species Reactivity

Equine

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 608714
View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant equine IL-1 beta /IL-1F2
Ala116-Ala268
Accession # Q28286

Specificity

Detects equine IL-1 beta /IL-1F2 in direct ELISAs. In direct ELISAs, 100% cross‑reactivity with recombinant canine, guinea pig, mouse, rat, and porcine IL‑1 beta is observed and 10‑50% cross‑reactivity with recombinant feline, human, rabbit, and rhesus IL‑1 beta is observed. Approximately 20% cross‑reactivity with recombinant mouse (rm) IL‑36 alpha and no cross‑reactivity with recombinant human (rh) IL‑36 alpha, rhIL‑36 gamma, rhIL‑1F10, rhIL‑36Ra, rmIL‑1Ra, rmIL‑18, or rmIL‑36 beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Equine IL‑1 beta /IL‑1F2 Antibody

IL-1 beta /IL-1F2 antibody in Equine PBMCs by Immunocytochemistry (ICC).

IL‑1 beta /IL‑1F2 in Equine PBMCs.

IL-1 beta /IL-1F2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Equine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB33401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Equine IL‑1 beta /IL‑1F2 Antibody

Application
Recommended Usage

Immunocytochemistry

20-30 µg/mL
Sample: Immersion fixed equine peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 1 review rated 5 using MAB33401 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta shares 65%-75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.

References

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Kato, H. et al. (1997) Vet. Immunol. Immunopathol. 48:221.
  6. Howard, R.D. et al. (1998) Am. J. Vet. Res. 59:704.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
  8. Kato, H. et al. (1996) Gene 177:11.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

Q28286

Additional IL-1 beta/IL-1F2 Products

Product Documents for Equine IL‑1 beta /IL‑1F2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Equine IL‑1 beta /IL‑1F2 Antibody

For research use only

Customer Reviews for Equine IL‑1 beta /IL‑1F2 Antibody (1)

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  • Equine IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Equine
    Verified Customer | Posted 03/12/2022
    Equine peripheral blood mononuclear cells (PBMCs)
    IL‑1 beta was detected in immersion fixed equine PBMCs at 25 ug/mL for 3 hours at room temperature.
    Equine IL‑1 beta /IL‑1F2 Antibody MAB33401

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