Measured by its ability to neutralize IFN‑ gamma inhibition of VSV-induced cytopathy in the CRFK feline kidney epithelial cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 5 ng/mL Recombinant Feline IFN‑ gamma.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN‑ gamma Inhibition of VSV-induced Cytopathy and Neutralization by Feline IFN‑ gamma Antibody.
Recombinant Feline IFN‑ gamma (Catalog # 764-FG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the CRFK feline kidney epithelial cell line in a dose-dependent manner (orange line). Inhibition of VSV activity elicited by Recombinant Feline IFN‑ gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF764). The ND50 is typically 1-3 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature feline IFN-gamma exists as a noncovalently linked homodimer of 20 25 kDa variably glycosylated subunits (3, 4). It shares 88% amino acid sequence identity with canine IFN-gamma, 72%‑78% with bovine, equine, and porcine IFN‑ gamma, and 40%‑62% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8).
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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