Feline IFN-gamma Antibody

Catalog # Availability Size / Price Qty
AF764
AF764-SP
IFN‑ gamma  Inhibition of VSV-induced Cytopathy and Neutralization by Feline IFN‑ gamma  Antibody.
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Citations (1)
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Feline IFN-gamma Antibody Summary

Species Reactivity
Feline
Specificity
Detects feline IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human, mouse, rat, and porcine IFN-gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant feline IFN-gamma
Gln24-Lys167
Accession # P46402
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Feline IFN-gamma (Catalog # 764-FG)
Neutralization
Measured by its ability to neutralize IFN‑ gamma inhibition of VSV-induced cytopathy in the CRFK feline kidney epithelial cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 5 ng/mL Recombinant Feline IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IFN‑ gamma  Inhibition of VSV-induced Cytopathy and Neutralization by Feline IFN‑ gamma  Antibody. View Larger

IFN‑ gamma  Inhibition of VSV-induced Cytopathy and Neutralization by Feline IFN‑ gamma Antibody.

Recombinant Feline IFN‑ gamma (Catalog # 764-FG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the CRFK feline kidney epithelial cell line in a dose-dependent manner (orange line). Inhibition of VSV activity elicited by Recombinant Feline IFN‑ gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF764). The ND50 is typically 1-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature feline IFN-gamma exists as a noncovalently linked homodimer of 20  25 kDa variably glycosylated subunits (3, 4). It shares 88% amino acid sequence identity with canine IFN-gamma, 72%‑78% with bovine, equine, and porcine IFN‑ gamma, and 40%‑62% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8).

References
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Argyle, D.J. et al. (1995) DNA Seq. 5:169.
  4. Schijns, V.E. et al. (1995) Immunogenetics 42:440.
  5. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  6. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  7. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  8. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  9. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  10. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Product Datasheets

Citation for Feline IFN-gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A targeted mutation within the feline leukemia virus (FeLV) envelope protein immunosuppressive domain to improve a canarypox virus-vectored FeLV vaccine.
    Authors: Schlecht-Louf G, Mangeney M, El-Garch H, Lacombe V, Poulet H, Heidmann T
    J Virol, 2014;88(2):992-1001.
    Species: Feline
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Isotype Controls

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Secondary Antibodies

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