Human 5-HT4 Antibody

Catalog # Availability Size / Price Qty
Detection 5-HT4 in HEK293 Human Cell Line Transfected with Human 5-HT4 and eGFP by Flow Cytometry
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Product Details
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Human 5-HT4 Antibody Summary

Species Reactivity
Detects human 5-HT4 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 631769
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human 5-HT4
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
 HEK293 human embryonic kidney cell line transfected with human 5-HT4 and eGFP
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection 5-HT4 in HEK293 Human Cell Line Transfected with Human 5-HT4 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human 5-HT4 and eGFP was stained with (A) Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) or (B) Mouse IgG2B isotype control antibody (IC0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunohistochemistry 5-HT4 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

5-HT4 in Human Brain. 5-HT4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT4

5-HT4 (5-hydroxytryptamine receptor 4), also known as HTR4 or 5HT4R, is an approximately 45 kDa multipass serotonin receptor that is expressed in the brain, gastrointestinal tract, bladder, vascular endothelial cells, atrium, kidney, and adrenal gland. It is involved in the regulation of learning, memory, anxiety, smooth muscle contraction, and adrenal corticosteroid secretion. Alternate splicing of human 5-HT4 generates multiple isoforms with either a 14 aa insertion in the second extracellular loop or a deletion or various substitutions in the C-terminal cytoplasmic region. Different isoforms show tissue specific expression patterns or increased activity in the absence of ligand. Human 5-HT4 shares 94% and 88% aa sequence identity with mouse and rat 5-HT4, respectively.

Long Name
5-Hydroxytryptamine Receptor 4
Entrez Gene IDs
3360 (Human)
Alternate Names
5HT4; 5-HT4; 5-HT-4; 5-HT4R; 5-hydroxytryptamine (serotonin) receptor 4,5-hydroxytryptamine receptor 4,5-HT45-hydroxytryptamine4 receptor; cardiac 5-HT4 receptor; HTR4; Serotonin receptor 4

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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