Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 631769
View all 5-HT4 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human 5-HT4

Specificity

Detects human 5-HT4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human 5-HT4 Antibody

Detection 5-HT4 in HEK293 Human Cell Line Transfected with Human 5-HT4 and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with human 5-HT4 and eGFP was stained with (A) Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) or (B) Mouse IgG2B isotype control antibody (IC0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.
5-HT4 antibody in Human Brain by Immunohistochemistry (IHC-P).

5-HT4 in Human Brain.

5-HT4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human 5-HT4 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample:  HEK293 human embryonic kidney cell line transfected with human 5-HT4 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: 5-HT4

5-HT4 (5-hydroxytryptamine receptor 4), also known as HTR4 or 5HT4R, is an approximately 45 kDa multipass serotonin receptor that is expressed in the brain, gastrointestinal tract, bladder, vascular endothelial cells, atrium, kidney, and adrenal gland. It is involved in the regulation of learning, memory, anxiety, smooth muscle contraction, and adrenal corticosteroid secretion. Alternate splicing of human 5-HT4 generates multiple isoforms with either a 14 aa insertion in the second extracellular loop or a deletion or various substitutions in the C-terminal cytoplasmic region. Different isoforms show tissue specific expression patterns or increased activity in the absence of ligand. Human 5-HT4 shares 94% and 88% aa sequence identity with mouse and rat 5-HT4, respectively.

Long Name

5-Hydroxytryptamine Receptor 4

Alternate Names

5HT4, HTR4

Entrez Gene IDs

3360 (Human)

Gene Symbol

HTR4

Additional 5-HT4 Products

Product Documents for Human 5-HT4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human 5-HT4 Antibody

For research use only

Citations for Human 5-HT4 Antibody

Customer Reviews for Human 5-HT4 Antibody

There are currently no reviews for this product. Be the first to review Human 5-HT4 Antibody and earn rewards!

Have you used Human 5-HT4 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies