AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2subunit in liver, while beta 2 is more abundant in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1092615
Loading...
Product Specifications
Immunogen
E. coli- derived recombinant human AMPKbeta2
Met1-Ile272
Accession # O43741
Met1-Ile272
Accession # O43741
Specificity
Detects recombinant human AMPK beta 2 protein in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human AMPK beta 2 Antibody
Detection of Human AMPK beta 2 by Western Blot.
Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human AMPK beta 2 Monoclonal Antibody (Catalog # MAB11639) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPK beta 2 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human AMPK beta 2 by Simple WesternTM.
Simple Western shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.5 mg/ml. A specific band was detected for AMPK beta 2 at approximately 38 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human AMPK beta 2 Monoclonal Antibody (Catalog # MAB11639). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.Applications for Human AMPK beta 2 Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: HEK293T human embryonic kidney cell line
Sample: HEK293T human embryonic kidney cell line
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line and U2OS human osteosarcoma
Sample: HepG2 human hepatocellular carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line and U2OS human osteosarcoma
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: AMPK beta 2
Long Name
AMP-activated Protein Kinase beta 2
Alternate Names
PRKAB2
Gene Symbol
PRKAB2
UniProt
Additional AMPK beta 2 Products
Product Documents for Human AMPK beta 2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human AMPK beta 2 Antibody
For research use only
Related Research Areas
Customer Reviews for Human AMPK beta 2 Antibody
There are currently no reviews for this product. Be the first to review Human AMPK beta 2 Antibody and earn rewards!
Have you used Human AMPK beta 2 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
