Human Angiopoietin-like Protein 1/ANGPTL1 Antibody

Catalog # Availability Size / Price Qty
MAB22041-100
MAB22041-SP
Human Angiopoietin-like Protein 1/ANGPTL1 Antibody in ELISA
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Human Angiopoietin-like Protein 1/ANGPTL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Angiopoietin-like Protein 1/ANGPTL1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 205811
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like Protein 1/ANGPTL1
Gly24-Asp491
Accession # O95841
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Angiopoietin‑like Protein 1/ANGPTL1 Monoclonal Antibody (Catalog # MAB10275).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Angiopoietin‑like Protein 1/ANGPTL1 ELISA Standard Curve. Recombinant Human Angiopoietin‑like Protein 1/ANGPTL1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Angiopoietin‑like Protein 1/ANGPTL1 Monoclonal Antibody (Catalog # MAB10275) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Angiopoietin‑like Protein 1/ANGPTL1 Monoclonal Antibody (Catalog # MAB22041) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 1/ANGPTL1

Angiopoietin-like 1 is a secreted protein belonging to the family of angiopoietins. It is expressed in highly vascularized adult tissues but is not an endothelial cell mitogen.

Entrez Gene IDs
9068 (Human); 72713 (Mouse); 679942 (Rat)
Alternate Names
ANG-3; ANG3angioarrestin; Angiopoietin like Protein 1; angiopoietin Y1; angiopoietin-3; angiopoietin-like 1; Angiopoietin-like Protein 1; angiopoietin-related protein 1; ANGPT3; ANGPTL1; AngY; AngY1; ARP1; ARP1angiopoietin 3; dJ595C2.2 (angiopoietin Y1); dJ595C2.2; KIAA0351; UNQ162

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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