Human Apolipoprotein H/ApoH Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human Apolipoprotein H/ApoH in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 517038
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Apolipoprotein H/ApoH
    Gly20-Cys345
    Accession # P02749
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation. PVDF membrane was probed with 1 µg/mL of Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Apolipoprotein H/ApoH at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human Apolipoprotein H/ApoH by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.2 mg/mL.
A specific band was detected for Apolipoprotein H/ApoH at approximately 67 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein H/ApoH
Apolipoprotein H (ApoH also known as beta 2-Glycoprotein 1/ beta 2-GPI) is a 50 kDa secreted monomeric glycoprotein member of the complement control superfamily of molecules. It is produced by hepatocytes and is bound to HDL particles. Circulating ApoH blocks the intrinsic clotting pathway and binds to exposed phospholipids on apoptotic cells. Mature human ApoH contains four Sushi repeats (aa 21‑260) and one C-terminal kringle domain (aa 261‑345) that binds heparin and phospholipids. At least two isoforms exist. One shows a 31 aa substitution for aa 140‑345, while a second represents a bioactive plasmin cleavage product that removes the C-terminal nine amino acids. Mature human ApoH shares 76% aa identity with mouse ApoH.
  • Entrez Gene IDs:
    350 (Human); 11818 (Mouse); 287774 (Rat)
  • Alternate Names:
    Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; APOH; apo-H; apolipoprotein H (beta-2-glycoprotein I); Apolipoprotein H; B2G1; B2GP1; B2GPI; Beta; beta(2)GPI; beta-2-glycoprotein 1; Beta-2-glycoprotein I; BG
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Streptococcal M1 protein constructs a pathological host fibrinogen network.
    Authors: Macheboeuf P, Buffalo C, Fu CY, Zinkernagel AS, Cole JN, Johnson JE, Nizet V, Ghosh P
    Nature, 2011;472(7341):64-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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