Human Apolipoprotein H/ApoH Antibody Summary
Accession # P02749
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation. PVDF membrane was probed with 1 µg/mL of Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Apolipoprotein H/ApoH at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human Apolipoprotein H/ApoH by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein H/ApoH at approximately 67 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein H/ApoH
Apolipoprotein H (ApoH also known as beta 2-Glycoprotein 1/ beta 2-GPI) is a 50 kDa secreted monomeric glycoprotein member of the complement control superfamily of molecules. It is produced by hepatocytes and is bound to HDL particles. Circulating ApoH blocks the intrinsic clotting pathway and binds to exposed phospholipids on apoptotic cells. Mature human ApoH contains four Sushi repeats (aa 21‑260) and one C-terminal kringle domain (aa 261‑345) that binds heparin and phospholipids. At least two isoforms exist. One shows a 31 aa substitution for aa 140‑345, while a second represents a bioactive plasmin cleavage product that removes the C-terminal nine amino acids. Mature human ApoH shares 76% aa identity with mouse ApoH.
Citation for Human Apolipoprotein H/ApoH Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Streptococcal M1 protein constructs a pathological host fibrinogen network.
Authors: Macheboeuf P, Buffalo C, Fu CY, Zinkernagel AS, Cole JN, Johnson JE, Nizet V, Ghosh P
Sample Types: Cell Lysates
Applications: Western Blot
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