Human APP/Protease Nexin II Pan Specific Antibody

Catalog # Availability Size / Price Qty
APP/Protease Nexin II in Human Brain.
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Product Details
Citations (2)
Supplemental Products

Human APP/Protease Nexin II Pan Specific Antibody Summary

Species Reactivity
Detects human APP695+1 and human APP770 in direct ELISAs and Western blots. This antibody recognizes a common epitope present on APP695+1 and APP770 found within the segments Leu18 - Arg288 or Pro365 - Arg411 of APP770 (Accession P05067).
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human APP 695+1 frameshift mutant
Accession # NP_958817
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human APP+1 Western Blot Standard (Catalog # WBC012)
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

APP/Protease Nexin II in Human Brain. APP/Protease Nexin II was detected in immersion fixed paraffin-embedded sections of human brain (Alzheimer's disease cortex) using Goat Anti-Human APP/Protease Nexin II Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1168) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APP

Amyloid precursor protein (APP) is a type I membrane protein with several human isoforms due to alternative splicing. APP-770, -751, and -733 contain a Kunitz protease inhibitor (KPI) domain (residue 291 - 342) and APP-695 does not. APP is a cell surface molecule with many functions. It can be processed proteolytically in two different pathways. In one pathway, beta - and gamma -secretase cleave at the  beta  site between residue 670 and 671 and the gamma site between residue 711 and 714 to produce beta ‑amyloid peptide (A beta 40 and A beta 42), a major component in plaques found in brains of patients with Alzheimer's disease (1). The other pathway involves alpha -secretase that cleaves residues between 687 and 688. It is antiamyloidogenic due to its benign character and the prevention of the A beta peptide formation (2). Soluble APP containing the KPI domain, also referred to as protease nexin II, is a potent inhibitor of serine proteases and may have additional functions. For example, it may regulate the contact face of blood coagulation and limit thrombosis specially in brain due to its localization and coagulation factor XI inhibiting activity (3, 4).

  1. Haass, C. (2004) EMBO J. 23:483.
  2. Lichtenthaler, S. F. and C. Haass (2004) J. Clin. Invest. 113:1384.
  3. Badellino, K.O. and P.N. Walsh (2000) Biochemistry 39:4769.
  4. Xu, F. et al. (2005) Proc. Natl. Acad. Sci USA. 102:18135.
Long Name
Amyloid Precursor Protein
Entrez Gene IDs
351 (Human); 11820 (Mouse)
Alternate Names
amyloid beta (A4) precursor protein-binding, family B, member 2; amyloid beta A4 precursor protein-binding family B member 2; Amyloid beta precursor protein; Amyloid beta; APP; beta Amyloid; Protease Nexin II

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Citations for Human APP/Protease Nexin II Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Absence of microglia promotes diverse pathologies and early lethality in Alzheimer's disease mice
    Authors: S Kiani Shab, S Morabito, EP Danhash, A McQuade, JR Sanchez, E Miyoshi, JP Chadarevia, C Claes, MA Coburn, J Hasselmann, J Hidalgo, KN Tran, AC Martini, W Chang Roth, J Pascual, E Head, DA Hume, C Pridans, H Davtyan, V Swarup, M Blurton-Jo
    Cell Reports, 2022-06-14;39(11):110961.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. A small molecule ApoE4-targeted therapeutic candidate that normalizes sirtuin 1 levels and improves cognition in an Alzheimer's disease mouse model
    Authors: J Campagna, P Spilman, B Jagodzinsk, D Bai, A Hatami, C Zhu, T Bilousova, M Jun, CJ Elias, J Pham, G Cole, MJ LaDu, ME Jung, DE Bredesen, V John
    Sci Rep, 2018-12-04;8(1):17574.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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