Human Arginase 1/ARG1 Antibody

Catalog # Availability Size / Price Qty
MAB5868
MAB5868-SP
Detection of Human Arginase 1/ARG1 by Western Blot.
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Human Arginase 1/ARG1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Arginase 1/ARG1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 658934
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Arginase 1/ARG1
Met1-Lys322
Accession # P05089
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunoprecipitation
25 µg/mL
Cell lysates spiked with Recombinant Human Arginase 1/ARG1 (Catalog # 5868-AR), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Arginase 1/ARG1 by Western Blot. View Larger

Detection of Human Arginase 1/ARG1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 1 µg/mL of Human Arginase 1/ARG1 Monoclonal Antibody (Catalog # MAB5868) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Arginase 1/ARG1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Arginase 1/ARG1 by Simple Western<sup>TM</sup>. View Larger

Detection of Human Arginase 1/ARG1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Arginase 1/ARG1 at approximately 42 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Arginase 1/ARG1 Monoclonal Antibody (Catalog # MAB5868). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.*Non-specific interaction with the 230kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is moderately active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Long Name
Liver-Type Arginase
Entrez Gene IDs
383 (Human); 11846 (Mouse); 29221 (Rat)
Alternate Names
AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; EC:3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Arginase 1/ARG1 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human Arginase 1/ARG1 Antibody
By Anonymous on 01/21/2022
Application: WB Sample Tested: Liver tissue Species: Human

Human Arginase 1/ARG1 Antibody
By Lucia Lisi on 05/19/2021
Application: ICC/IF Sample Tested: MICROGLIA Species: Human