Human Atrial Natriuretic Peptide/ANP Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Atrial Natriuretic Peptide/ANP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BNP, rhANF, and rhPTCH-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Atrial Natriuretic Peptide/ANP
    Asn26-Tyr151
    Accession # P01160
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    3-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Atrial Natriuretic Peptide/ANP by Western Blot. Western blot shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyoctyes (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Atrial Natriuretic Peptide/ANP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Atrial Natriuretic Peptide/ANP at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Atrial Natriuretic Peptide/ANP in BG01V Human Embryonic Stem Cells. Atrial Natriuretic Peptide/ANP was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Goat Anti-Human Atrial Natriuretic Peptide/ANP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3366) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunohistochemistry
Atrial Natriuretic Peptide/ANP in Human Prostate. Atrial Natriuretic Peptide/ANP was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Atrial Natriuretic Peptide/ANP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3366) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Atrial Natriuretic Peptide/ANP by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for Atrial Natriuretic Peptide/ANP at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Atrial Natriuretic Peptide/ANP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3366) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Atrial Natriuretic Peptide/ANP

ANP (Atril Natriuetic Peptide; also known as gamma -ANP, CDP/cardiodilatin-related peptide and CDD-ANF) is a secreted member of the natriuretic peptide family of molecules. It is expressed in multiple cell types, including atrial myocardiocytes, macrophages and select hypothalamic neurons. The C-terminus of ANP (or ANF) is reported to have a wide range of activities. It is best known to promote sodium and water excretion, but is also known to inhibit aldosterone and vasopressin secretion, while promoting testosterone and LH release. Human (pro)ANP is synthesized as a 153 amino acid (aa) preproprecursor that contains a 25 aa signal sequence, cardiodilatin-related peptide (aa 26-55), a prosequence (aa 56-123) and a C-terminal atrial natriuretic factor motif (aa 124-151) that may or may not be accompanied by a dibasic Arg fragment. Although multiple enzymes are reported to be active on proANP, transmembrane Corin would appear to be central to normal extracellular proteolytic processing and the generation of ANP. Notably, and depending upon the tissue, proANP can also be cleaved at alternate sites, including Ala120 and Arg126. There is at least one isoform variant that contains a 12 aa addition to the C-terminus of the proprecursor, resulting in an ANP that is 40 aa in length. Over aa 26-151 (proANP), human proANP shares 84% aa sequence identity with mouse proANP.

  • Entrez Gene IDs:
    4878 (Human); 230899 (Mouse); 24602 (Rat)
  • Alternate Names:
    ANF; ANP; ANPatriopeptin; ATFB6; atrial natriuretic factor; Atrial Natriuretic Peptide; Cardionatrin; CDD-ANF; natriuretic peptide A; natriuretic peptide precursor A; NPPA; PND; PNDcardionatrin; prepronatriodilatin
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