Human beta -Catenin Antibody

(4 citations)
(4 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human  beta -Catenin in direct ELISAs.
  • Source
    Monoclonal Mouse IgG3 Clone # 196618
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human beta -Catenin
    aa 2-781
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human beta -Catenin
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
beta ‑Catenin in SW480 Human Cell Line.

beta -Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human beta ‑Catenin Monoclonal Antibody (Catalog # MAB13291) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry
beta ‑Catenin in Human Pancreas. beta ‑Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human beta ‑Catenin Monoclonal Antibody (Catalog # MAB13291) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Catenin
Beta-Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as as transcriptional co-activator in the nucleus and is also involved the canonical Wnt signal transduction pathways.
  • Entrez Gene IDs:
    1499 (Human); 12387 (Mouse); 84353 (Rat)
  • Alternate Names:
    bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells.
    Authors: Vicente C, Lima M, Nader H, Toma L
    J Exp Clin Cancer Res, 2015;34(0):25.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
    Authors: Riordan D, Varma S, West R, Brown P
    PLoS ONE, 2015;10(7):e0128975.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
    Authors: Moore RN, Cherry JF, Mathur V
    Stem Cells Dev., 2012;21(1):30-41.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Survivin in the human hair follicle.
    Authors: Botchkareva NV, Kahn M, Ahluwalia G, Shander D
    J. Invest. Dermatol., 2007;127(2):479-82.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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Isotype Controls
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Mouse IgG3 Isotype Control

Ctrl MAB007 3  
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Average Rating: 4.8 (Based on 4 reviews)

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Read what people are saying who have used Human beta -Catenin Antibody.

We have 4 reviews tested in 1 species: Human.
We have 4 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human  beta -Catenin Antibody MAB13291
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Excellent
 ICC/IF Human Anonymous 11/03/2017
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Image Details
Immunocytochemistry/Immunofluorescence Human  beta -Catenin Antibody MAB13291
Immunocytochemistry/Immunofluorescence: Human beta -Catenin Antibody [MAB13291]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHCT-116 human colorectal carcinoma cell line
SpeciesHuman
Western Blot Human beta ‑Catenin Antibody MAB13291
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Excellent
 WB Human Anonymous 10/26/2015
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Western Blot Human beta ‑Catenin Antibody MAB13291
Western Blot: Human beta ‑Catenin Antibody [MAB13291]

Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Sensitive
Buffer: 5% milk in TBST
Dilution: 1/500
Immunocytochemistry/Immunofluorescence Human beta ‑Catenin Antibody MAB13291
Enlarge
Excellent
 ICC/IF Human Anonymous 10/26/2015
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Image Details
Immunocytochemistry/Immunofluorescence Human beta ‑Catenin Antibody MAB13291
Immunocytochemistry/Immunofluorescence: Human beta ‑Catenin Antibody [MAB13291]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHuman cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100
Immunocytochemistry/Immunofluorescence Human beta ‑Catenin Antibody MAB13291
Enlarge
Very Good
 ICC/IF Human Anonymous 10/26/2015
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Image Details
Immunocytochemistry/Immunofluorescence Human beta ‑Catenin Antibody MAB13291
Immunocytochemistry/Immunofluorescence: Human beta ‑Catenin Antibody [MAB13291]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHuman cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSpecificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/62.5

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