Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 516505
View all beta-Synuclein Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human beta -Synuclein
Gly47-Glu120
Accession # Q16143

Specificity

Detects human beta -Synuclein in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human alpha -Synuclein is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human beta -Synuclein Antibody

Detection of Human beta -Synuclein antibody by Western Blot.

Detection of Human beta -Synuclein by Western Blot.

Western blot shows lysates of human hippocampal tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta -Synuclein Monoclonal Antibody (Catalog # MAB5528) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for beta -Synuclein at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of beta -Synuclein in Human Hippocampus.

beta -Synuclein was detected in immersion fixed paraffin-embedded sections of Human Hippocampus using Mouse Anti-Human beta -Synuclein Monoclonal Antibody (Catalog # MAB5528) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human beta -Synuclein Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus

Western Blot

2 µg/mL
Sample: Human hippocampal tissue

Reviewed Applications

Read 1 review rated 5 using MAB5528 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-Synuclein

beta -Synuclein  is a 19 kDa, 134 amino acid (aa) cytoplasmic phosphoprotein that is a member of a family of small, highly conserved proteins, including synucleins alpha, beta, and gamma (synoretin), localized predominantly in presynaptic nerve terminals in the brain. beta -Synuclein  associates with alpha -Synuclein, preventing it from aggregating. Abnormal alpha -Synuclein aggregates form filamentous inclusions (Lewy bodies) in neurodegenerative diseases including Parkinson's disease. Within the region used as an immunogen, human SNCB shares 99% aa identity with mouse and rat beta -Synuclein.

Alternate Names

SNCB, Synuclein-beta

Entrez Gene IDs

6620 (Human); 104069 (Mouse); 113893 (Rat)

Gene Symbol

SNCB

UniProt

Q16143 (Human)

Additional beta-Synuclein Products

Product Documents for Human beta -Synuclein Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human beta -Synuclein Antibody

For research use only

Customer Reviews for Human beta -Synuclein Antibody (1)

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  • Human beta-Synuclein Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 05/16/2022
    Human beta -Synuclein Antibody MAB5528

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Protocols

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FAQs

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