Human BMP-4 Antibody
Human BMP-4 Antibody Summary
Accession # P12644
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody. Recombinant Human BMP-4 (Catalog # 314-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF757). The ND50 is typically 0.5-2.0 µg/mL in the presence of heparin (2 µg/mL).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 - 5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfide-linked heterodimer (7 - 9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR‑IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13 - 15).
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Citations for Human BMP-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of Dedifferentiating Muscle Cells during Newt Limb Regeneration
Authors: I Wagner, H Wang, PM Weissert, WL Straube, A Shevchenko, M Gentzel, G Brito, A Tazaki, C Oliveira, T Sugiura, A Shevchenko, A Simon, DN Drechsel, EM Tanaka
Dev. Cell, 2017-03-27;40(6):608-617.e6.
Applications: Immunoprecipitation, Western Blot
A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering.
Authors: Mazaki T, Shiozaki Y, Yamane K, Yoshida A, Nakamura M, Yoshida Y, Zhou D, Kitajima T, Tanaka M, Ito Y, Ozaki T, Matsukawa A
Sci Rep, 2014-03-25;4(0):4457.
Sample Types: Whole Cells
Malignant transformation of multipotent muscle-derived cells by concurrent differentiation signals.
Authors: Pollett JB, Corsi KA, Weiss KR, Cooper GM, Barry DA, Gharaibeh B, Huard J
Stem Cells, 2007-06-14;25(9):2302-11.
Sample Types: Whole Tissue
Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
Authors: Maeshima A, Vaughn DA, Choi Y, Nigam SK
Dev. Biol., 2006-04-27;295(2):473-85.
Sample Types: Whole Tissue
VEGF-A signaling through Flk-1 is a critical facilitator of early embryonic lung epithelial to endothelial crosstalk and branching morphogenesis.
Authors: Del Moral PM, Sala FG, Tefft D, Shi W, Keshet E, Bellusci S, Warburton D
Dev. Biol., 2005-12-22;290(1):177-88.
Sample Types: Tissue Homogenates
Applications: Western Blot
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