Human C-Reactive Protein/CRP Antibody

Catalog # Availability Size / Price Qty
MAB17073-100
MAB17073-SP
Human C-Reactive Protein/CRP Antibody in ELISA
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Human C-Reactive Protein/CRP Antibody Summary

Species Reactivity
Human
Specificity
Detects human C‑Reactive Protein/CRP in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 987313
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human C‑Reactive Protein/CRP
Phe17-Pro224
Accession # P02741
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human C‑Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17072).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human C-Reactive Protein/CRP DuoSet ELISA Kit (Catalog # DY1707) for convenient development of a sandwich ELISA or the Human C-Reactive Protein/CRP Quantikine ELISA Kit (Catalog # DCRP00) for a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human C‑Reactive Protein/CRP ELISA Standard Curve. Recombinant Human C-Reactive Protein/CRP protein was serially diluted 2-fold and captured by Mouse Anti-Human C-Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17073) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human C-Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17072) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C-Reactive Protein/CRP

CRP is a secreted protein that belongs to the Pentraxin family. It circulates as a non-covalent homopentamer. The CRP concentration in human plasma is highly increased during acute phase response to tissue injury or infection.

Entrez Gene IDs
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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