Human C-Reactive Protein/CRP Antibody Summary
Accession # P02741
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human C‑Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17072).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human C-Reactive Protein/CRP DuoSet ELISA Kit (Catalog # DY1707) for convenient development of a sandwich ELISA or the Human C-Reactive Protein/CRP Quantikine ELISA Kit (Catalog # DCRP00) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human C‑Reactive Protein/CRP ELISA Standard Curve. Recombinant Human C-Reactive Protein/CRP protein was serially diluted 2-fold and captured by Mouse Anti-Human C-Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17073) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human C-Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17072) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C-Reactive Protein/CRP
CRP is a secreted protein that belongs to the Pentraxin family. It circulates as a non-covalent homopentamer. The CRP concentration in human plasma is highly increased during acute phase response to tissue injury or infection.
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