Human Calbindin D Antibody

Catalog # Availability Size / Price Qty
MAB3320
MAB3320-SP
Human Calbindin D Antibody in
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Product Details
Citations (1)
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Human Calbindin D Antibody Summary

Species Reactivity
Human
Specificity
Detects human Calbindin D in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human S100P.
Source
Monoclonal Mouse IgG2B Clone # 401025
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Calbindin D
Met1-Asn261
Accession # P05937
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Calbindin D in Rat Brainstem. View Larger

Calbindin D in Rat Brainstem. Calbindin D was detected in perfusion fixed paraffin-embedded sections of rat brainstem (medulla) using 25 µg/mL Mouse Anti-Human Calbindin D Monoclonal Antibody (Catalog # MAB3320) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Calbindin D by Simple Western<sup>TM</sup>. View Larger

Detection of Human Calbindin D by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Calbindin D Monoclonal Antibody (Catalog # MAB3320). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry Calbindin D in Rat Brain. View Larger

Calbindin D in Rat Brain. Calbindin D was detected in perfusion fixed frozen sections of rat brain (hypothalamus) using 25 µg/mL Mouse Anti-Human Calbindin D Monoclonal Antibody (Catalog # MAB3320) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Calbindin D in Human Brain. View Larger

Calbindin D in Human Brain. Calbindin D was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 25 µg/mL Mouse Anti-Human Calbindin D Monoclonal Antibody (Catalog # MAB3320) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calbindin D

Calbindin D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin D is widely expressed and regulates calcium homeostasis. Calbindin D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium. Human, mouse, and rat Calbindin D share 99% amino acid sequence identity.

Long Name
Vitamin D-dependent Calcium-Binding Protein
Entrez Gene IDs
795 (Human); 12309 (Mouse); 24249 (Rat)
Alternate Names
CABP; CABP1; CABP28K; CABP9K; Calbindin 3; Calbindin D; Calbindin-D; Calbindin-D9K; S100G

Product Datasheets

Citation for Human Calbindin D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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