Human Carbonic Anhydrase IV/CA4 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
    10% cross‑reactivity with recombinant human (rh) CA1, rhCA2, and recombinant mouse CA4 is observed. Does not cross-react with rhCA3, 8, 9, 10, 12, 13, or 14.
  • Source
    Monoclonal Mouse IgG2B Clone # 310413
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IV/CA4
    Ala19-Lys283
    Accession # P22748
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human colon
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase IV (Catalog # 2186-CA), see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Carbonic Anhydrase IV/CA4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase IV/CA4 Monoclonal Antibody (Catalog # MAB2186) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase IV/CA4

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA4 is a
GPI‑anchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO2 taste sensor (2). In addition, a genetic mutation (Arg 14 to Trp in the signal peptide) of CA4 was found to co‑segregate with the RP17 form of retinitis pigmentosa in two large families and was not found in 36 unaffected family members or 100 controls (3).

  • References:
    1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
    2. Chandrashekar, J. et al. (2009) Science 326:443.
    3. Rebello, G. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6617.
  • Entrez Gene IDs:
    762 (Human); 12351 (Mouse)
  • Alternate Names:
    CA4; CAIV; CA-IV; Car4; Carbonate dehydratase IV; carbonic anhydrase 4; Carbonic Anhydrase IV; carbonic anhydrase IVRP17; carbonic dehydratase IV; EC 4.2.1.1; retinitis pigmentosa 17 (autosomal dominant); RP17
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Intracellular and extracellular carbonic anhydrases cooperate non-enzymatically to enhance activity of monocarboxylate transporters.
    Authors: Klier, Michael, Andes, Fabian T, Deitmer, Joachim, Becker, Holger M
    J Biol Chem, 2014;289(5):2765-75.
    Species: Xenopus
    Sample Type: Cell Lysates
    Application: WB
  2. Transport activity of the high-affinity monocarboxylate transporter MCT2 is enhanced by extracellular carbonic anhydrase IV but not by intracellular carbonic anhydrase II.
    Authors: Klier M, Schuler C, Halestrap AP, Sly WS, Deitmer JW, Becker HM
    J. Biol. Chem., 2011;286(31):27781-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Studies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium.
    Authors: Shahidullah M, To CH, Pelis RM, Delamere NA
    Invest. Ophthalmol. Vis. Sci., 2009;50(4):1791-800.
    Species: Porcine
    Sample Type: Whole Cells
    Application: ICC
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