Human Carboxypeptidase M Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Carboxypeptidase M by Western Blot. Western blot shows lysates of human placenta tissue and human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Carboxypeptidase M Monoclonal Antibody (Catalog # MAB7457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carboxypeptidase M at approximately 58-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry View Larger

Carboxypeptidase M in Human Placenta. Carboxypeptidase M was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Carboxypeptidase M Monoclonal Antibody (Catalog # MAB7457) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to plasma membranes of decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Background: Carboxypeptidase M

Carboxypeptidase M (CPM) is a 50‑65 kDa monomer that belongs to the regulatory CPN/E subfamily, peptidase M14 family of enzymes. It is widely expressed, being found on macrophages, fibroblasts, endothelial cells, oligodendrocytes and Schwann cells, dendritic cells, osteoblasts and bronchial epithelium. Carboxypeptidase M is a GPI‑linked glycoprotein that is best known as a peptidase that cleaves basic amino acids (aa) from the carboxyterminal of a number of peptides, including EGF and bradykinin. It is also known to bind to apparent substrates and undergo a conformational change that links it with the kinin B1 GPCR, initiating signal transduction. Mature human Carboxypeptidase M is 406 aa in length (aa 18-423). It contains one large enzymatic region (aa 19-310) and two critical glutamic acid residues at Glu260 and Glu264. Like many GPI‑linked proteins, Carboxypeptidase M undergoes solubilization and is reportedly found in urine and amniotic fluid. Over aa 18-423 (mature Carboxypeptidase M), human Carboxypeptidase M shares 86% aa sequence identity with mouse Carboxypeptidase M.

Product Datasheets

Related Research Areas

• Metalloproteases and Regulators
• Monocyte Markers
• Search Bioprocessing Enzymes