Human Caspase-1 Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Caspase-1 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 661228
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant human Caspase-1
    Asn120-Asp297
    Accession # P29466
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Caspase‑1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase‑1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
Caspase‑1 in THP‑1 Human Cell Line. Caspase‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-1

Caspase-1, also known as IL-1 beta -converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.

  • Entrez Gene IDs:
    834 (Human)
  • Alternate Names:
    CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta; caspase 1, apoptosis-related cysteine protease (interleukin 1, beta; Caspase1; Caspase-1; EC 3.4.22; EC 3.4.22.36; ICE; ICEP45; IL-1 beta-converting enzyme; IL1BC CASP1 nirs variant 1; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1-B converting enzyme; interleukin 1-beta convertase; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. AIM2 Inflammasome Is Critical for Influenza-Induced Lung Injury and Mortality
    Authors: H Zhang, J Luo, JF Alcorn, K Chen, S Fan, J Pilewski, A Liu, W Chen, JK Kolls, J Wang
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity
    Authors: JS Bednash, N Weathingto, J Londino, M Rojas, DL Gulick, R Fort, S Han, AC McKelvey, BB Chen, RK Mallampall
    Nat Commun, 2017;8(0):15203.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity
    Authors: JS Bednash, N Weathingto, J Londino, M Rojas, DL Gulick, R Fort, S Han, AC McKelvey, BB Chen, RK Mallampall
    Nat Commun, 2017;8(0):15203.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Monocyte Caspase-1 Is Released in a Stable, Active High Molecular Weight Complex Distinct from the Unstable Cell Lysate-Activated Caspase-1.
    Authors: Shamaa O, Mitra S, Gavrilin M, Wewers M
    PLoS ONE, 2015;10(11):e0142203.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: IP
  5. Age-related increases in amyloid beta and membrane attack complex: evidence of inflammasome activation in the rodent eye.
    Authors: Zhao T, Gao J, Van J, To E, Wang A, Cao S, Cui J, Guo J, Lee M, McGeer P, Matsubara J
    J Neuroinflammation, 2015;12(0):121.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  6. Propionibacterium acnes Induces IL-1beta secretion via the NLRP3 inflammasome in human monocytes.
    Authors: Qin M, Pirouz A, Kim M, Krutzik S, Garban H, Kim J
    J Invest Dermatol, 2014;134(2):381-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2A PE-conjugated Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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