Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 724810
View all Catalase Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Catalase
Met1-Leu527
Accession # P04040

Specificity

Detects human Catalase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Catalase or recombinant human Serpin C1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Catalase Antibody

Detection of Human Catalase antibody by Western Blot.

Detection of Human Catalase by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Catalase antibody by Simple WesternTM.

Detection of Human Catalase by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Catalase at approximately 61 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Catalase antibody in HL-60 Human Cell Line by Immunocytochemistry (ICC).

Catalase in HL‑60 Human Cell Line.

Catalase was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to peroxisomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Western Blot Shows Human Catalase Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Catalase Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Catalase Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HL-60 human acute promyelocytic leukemia cell line

Knockout Validated

Catalase is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Catalase knockout HeLa cell line.

Simple Western

5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Catalase

Cells have evolved complex mechanisms to maintain redox balance and defend against oxidative stress. Catalase is a tetrameric enzyme comprised of four 60 kDa subunits. Catalase is typically localized in the peroxisome where it functions as an antioxidant, protecting cells from damage due to oxidative stress. Catalase converts reactive oxygen species, such as H2O2, into water and O2. Human Catalase shares 89% homology to mouse and rat Catalase. The cells redox environment can serve as an important signaling switch or trigger to initiate a number of cellular processes, including gene expression, differentiation, proliferation and apoptosis.

Alternate Names

Cas1, CAT, Cs-1

Entrez Gene IDs

847 (Human); 12359 (Mouse); 24248 (Rat)

Gene Symbol

CAT

UniProt

P04040

Additional Catalase Products

Product Documents for Human Catalase Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Catalase Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Oxidative Stress

Citations for Human Catalase Antibody

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Protocols

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