Human CCL26/Eotaxin-3 Antibody

Catalog # Availability Size / Price Qty
AF653
AF653-SP
Human CCL26/Eotaxin-3 Antibody in Western Blot
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Product Details
Citations (3)
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Human CCL26/Eotaxin-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects CCL26/Eotaxin‑3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Eotaxin, recombinant mouse (rm) Eotaxin, rhEotaxin-2, and rmEotaxin-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL26/Eotaxin‑3
Thr24-Leu94
Accession # Q9Y258
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human colon subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Neutralization
Measured by its ability to neutralize CCL26/Eotaxin‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 4-24 µg/mL in the presence of 1 µg/mL Recombinant Human CCL26/Eotaxin‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human CCL26/Eotaxin‑3 by Western Blot. View Larger

Detection of Human CCL26/Eotaxin‑3 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL‑4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL26/Eotaxin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF653) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL26/
Eotaxin‑3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio  by Human CCL26/Eotaxin‑3 Antibody. View Larger

Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralizatio  by Human CCL26/Eotaxin‑3 Antibody. Recombinant Human CCL26/Eotaxin‑3 (Catalog # 346‑E3) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL26/Eotaxin‑3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL26/Eotaxin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF653). The ND50 is typically 4‑24 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL26/Eotaxin-3

Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin‑3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4.

Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. Recombinant Eotaxin-3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. Recombinant Eotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin-3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3-transfected cell line.

References
  1. Gou, R.F. et al. (1999) Genomics 58:313.
  2. Kitamura, M. et al. (1999) J. Biol. Chem. 274:27975.
  3. Shinkai, A. et al. (1999) J. Immunol. 163:1602.
Entrez Gene IDs
10344 (Human)
Alternate Names
CC chemokine IMAC; C-C motif chemokine 26; CCL26; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin3; Eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4a; MIP-4alpha; SCYA26; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; Small-inducible cytokine A26; TSC-1Thymic stroma chemokine-1

Product Datasheets

Citations for Human CCL26/Eotaxin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Proton pump inhibitors decrease eotaxin-3 expression in the proximal esophagus of children with esophageal eosinophilia.
    Authors: Park, Jason Y, Zhang, Xi, Nguyen, Nathalie, Souza, Rhonda F, Spechler, Stuart J, Cheng, Edaire
    PLoS ONE, 2014;9(7):e101391.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. High Levels of CCL26 in Blister Fluid and Sera of Patients with Bullous Pemphigoid.
    Authors: Kagami S, Kai H, Kakinuma T
    J. Invest. Dermatol., 2012;132(1):249-51.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
    Authors: Rump L, Mattey D, Kehoe O, Middleton J
    Cytokine, 0;97(0):133-140.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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