Human CCR5 Antibody

(10 citations)   
  • Species Reactivity
    Human
  • Specificity
    Can be used to detect CCR5 present on stimulated human PBMCs only after cells are fixed with 2% formaldehyde. It does not detect CCR5 on unfixed human PBMCs. It does not cross-react with CCR1, CCR2, or CCR3. For additional information regarding epitope specificity for this antibody and other R&D Systems anti-human CCR5 antibodies, see Lee, B. et al., 1999, J. Biol. Chem. 274:9617.
  • Source
    Monoclonal Mouse IgG2B Clone # 45523
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CCR5
    Met1-Leu352
    Accession # P51681
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CCR5 in Human Dorsal Root Ganglia. CCR5 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using 25 µg/mL Human CCR5 Monoclonal Antibody (Catalog # MAB181) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

  • Entrez Gene IDs:
    1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
  • Alternate Names:
    C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
    Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J
    Nature, 2013;493(7430):51-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Primary peripheral T-cell lymphoma, not otherwise specified of the thyroid with autoimmune thyroiditis.
    Authors: Yoshida N, Nishikori M, Izumi T, Imaizumi Y, Sawayama Y, Niino D, Tashima M, Hoshi S, Ohshima K, Shimoyama M, Seto M, Tsukasaki K
    Br J Haematol, 2013;161(2):214-23.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  3. Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
    Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
    Am. J. Transplant., 2008;8(7):1512-22.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes.
    Authors: Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M
    AIDS Res. Hum. Retroviruses, 2006;22(11):1152-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro.
    Authors: Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N
    Am. J. Pathol., 2006;169(6):2094-103.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. CCR5 expression and CC chemokine levels in idiopathic pulmonary fibrosis.
    Authors: Capelli A, Di Stefano A, Gnemmi I, Donner CF
    Eur. Respir. J., 2005;25(4):701-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2.
    Authors: Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F
    Cell. Signal., 2005;17(3):355-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
    Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
    Eur. J. Immunol., 2004;34(12):3485-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Chemokines and chemokine receptors in inflammatory demyelinating neuropathies: a central role for IP-10.
    Authors: Kieseier BC, Tani M, Mahad D, Oka N, Ho T, Woodroofe N, Griffin JW, Toyka KV, Ransohoff RM, Hartung HP
    Brain, 2002;125(0):823-34.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
    Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
    J. Biol. Chem., 1999;274(14):9617-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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Isotype Controls
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