Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 150503R-FAB2-TEV/His
View all CCR7 Primary Antibodies »

Product Specifications

Immunogen

Human CCR7 transfectants
Met1-Pro378
Accession # AAA58615

Specificity

Stains human CCR7 in flow cytometry

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CCR7 Antibody

Detection of CCR7 in Human Lymphocytes by Flow Cytometry with anti-Mouse Secondary Antibody.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CCR7 FAB2 Recombinant Monoclonal Antibody (Catalog # MAB197R-FAB2) or (B) Mouse IgG2A isotype control antibody (MAB003), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Detection of CCR7 in Human Lymphocytes by Flow Cytometry with anti-His antibody.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CCR7 FAB2 Recombinant Monoclonal Antibody (Catalog # MAB197R-FAB2) or (B) Mouse IgG2A isotype control antibody (MAB003), followed by PE-conjugated Anti-His Antibody (IC050P) and Mouse anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human CCR7 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: PBMC lymphocytes

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Alternate Names

BLR2, CC-CKR-7, CCR7, CD197, CDw197, CMKBR7, EBI1

Entrez Gene IDs

1236 (Human); 12775 (Mouse); 287673 (Rat)

Gene Symbol

CCR7

UniProt

AAA58615

Additional CCR7 Products

Product Documents for Human CCR7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCR7 Antibody

For research use only

Citations for Human CCR7 Antibody

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