Human CCR7 Antibody Summary
Accession # AAA58615
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCR7 in Human PBMCs by Flow Cytomtetry. Human peripheral blood mononuclear cells (PBMCs) were stained with PE-conjugated Mouse anti-Human CD4 Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197R) or (B) Mouse IgG2Aisotype control antibody (Catalog # MAB003) followed by APC-conjugated anti-mouse IgG secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Chemotaxis Induced by CCL19/MIP-3 beta and Neutral-ization by Human CCR7 Antibody. Recombinant Human CCL19/MIP-3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197R). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.
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