Human CD11b/Integrin alpha M Antibody

Catalog # Availability Size / Price Qty
MAB16992
MAB16992-SP
Product Details
Citations (5)
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Human CD11b/Integrin alpha M Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD11b/Integrin alpha M in direct ELISAs and Western blots. In Western blots no cross-reactivity with recombinant mouse (rm) Integrins alpha 2, alpha 3, alpha 4, alpha E or rhIntegrins alpha 5, alpha V, beta 1, beta 2, beta 3, or beta 6.
Source
Monoclonal Mouse IgG1 Clone # 238439
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alpha M
Phe17-Asn1105
Accession # NP_001139280
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Integrin  alpha M/CD11b

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

Product Datasheets

Citations for Human CD11b/Integrin alpha M Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Differential expression of adenine nucleotide converting enzymes in mitochondrial intermembrane space: a potential role of adenylate kinase isozyme 2 in neutrophil differentiation.
    Authors: Tanimura, Ayako, Horiguchi, Taigo, Miyoshi, Keiko, Hagita, Hiroko, Noma, Takafumi
    PLoS ONE, 2014;9(2):e89916.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
    BMC Neurosci, 2012;13(0):50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. 1,25-Dihydroxyvitamin D3 promotes a sustained LPS-induced NF-kappaB-dependent expression of CD55 in human monocytic THP-1 cells.
    Authors: Izban M, Nowicki B, Nowicki S
    PLoS ONE, 2012;7(11):e49318.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms.
    Authors: Nakayama H, Yoshizaki F, Prinetti A, Sonnino S, Mauri L, Takamori K, Ogawa H, Iwabuchi K
    J. Leukoc. Biol., 2007;83(3):728-41.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Protein-tyrosine kinase Syk is required for pathogen engulfment in complement-mediated phagocytosis.
    Authors: Shi Y, Tohyama Y, Kadono T, He J, Shahjahan Miah SM, Hazama R, Tanaka C, Tohyama K, Yamamura H
    Blood, 2006;107(11):4554-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Secondary Antibodies

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