Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, COMET

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 238439
View all CD11b/Integrin alpha M Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alpha M
Phe17-Asn1105
Accession # NP_001139280

Specificity

Detects human CD11b/Integrin alpha M in direct ELISAs and Western blots. In Western blots no cross-reactivity with recombinant mouse (rm) Integrins alpha 2, alpha 3, alpha 4, alpha E or rhIntegrins alpha 5, alpha V, beta 1, beta 2, beta 3, or beta 6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD11b/Integrin alpha M Antibody (238439)

Detection of CD11b/Integrin alpha M in Human Colon Cancer via seqIF™ staining on COMET™

CD11b/Integrin alpha M was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16992) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of CD11b/Integrin alpha M in Human Spleen via Multiplex Immunofluorescence staining on COMET™

CD11b/Integrin alpha M was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (MAB16992) at 15 μg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of CD11b/Integrin alpha M in Human Tonsil via seqIF™ staining on COMET™

CD11b/Integrin alpha M was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16992) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of CD11b/Integrin alpha M in Human Spleen.

CD11b/Integrin alpha M was detected in formalin fixed paraffin embedded sections of human spleen using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16992) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with Hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of CD11b/Integrin alpha M by Western Blot

Detection of CD11b/Integrin alpha M by Western Blot

Triple-cell co-culture provides pure cellular niches for macrophages, vascular endothelial and vascular smooth muscle cells. (A) Prior to completion of co-cultures via the addition of THP-1 cells, EC/SMC coated transwell inserts were analyzed via immunofluorescent microscopy for CD31 (red) and alpha SMA (green). Scale Bars = 40 μm. Following completion of EC/SMC/THP-1 co-cultures using both 0.4 μm (B) and 3 μm (C) transwell inserts, cells were isolated from each cellular niche and analyzed by immunoblotting for eNOS, alpha SMA, and CD11b. n = 3 co-cultures. (D) SMC were cultured on the underside of 3 μm transwell inserts and 3 days later analyzed by immunofluorescent microscopy for the expression of alpha SMA (green). (E) ECs or SMCs were cultured on 0.4 μm inserts until confluent. They were then mechanically disrupted and analyzed for viability using trypan blue exclusion. n = 6 cultures. Scale Bar = 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31068936), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD11b/Integrin alpha M by Western Blot

Detection of CD11b/Integrin alpha M by Western Blot

Triple-cell co-culture provides pure cellular niches for macrophages, vascular endothelial and vascular smooth muscle cells. (A) Prior to completion of co-cultures via the addition of THP-1 cells, EC/SMC coated transwell inserts were analyzed via immunofluorescent microscopy for CD31 (red) and alpha SMA (green). Scale Bars = 40 μm. Following completion of EC/SMC/THP-1 co-cultures using both 0.4 μm (B) and 3 μm (C) transwell inserts, cells were isolated from each cellular niche and analyzed by immunoblotting for eNOS, alpha SMA, and CD11b. n = 3 co-cultures. (D) SMC were cultured on the underside of 3 μm transwell inserts and 3 days later analyzed by immunofluorescent microscopy for the expression of alpha SMA (green). (E) ECs or SMCs were cultured on 0.4 μm inserts until confluent. They were then mechanically disrupted and analyzed for viability using trypan blue exclusion. n = 6 cultures. Scale Bar = 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31068936), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CD11b/Integrin alpha M Antibody (238439)

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

3-25 µg/mL
Sample: Human Spleen Tissue

Multiplex Immunofluorescence

15 µg/mL
Sample: Immersion fixed paraffin embedded human spleen, human tonsil, and human colon cancer tissue

Western Blot

1 µg/mL
Sample: Recombinant Human Integrin  alpha M/CD11b

Reviewed Applications

Read 1 review rated 5 using MAB16992 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

Alternate Names

CD11b, Integrin alpha M, ITGAM

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

UniProt

NP_001139280

Additional CD11b/Integrin alpha M Products

Product Documents for Human CD11b/Integrin alpha M Antibody (238439)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD11b/Integrin alpha M Antibody (238439)

For research use only

Citations for Human CD11b/Integrin alpha M Antibody (238439)

Customer Reviews for Human CD11b/Integrin alpha M Antibody (238439) (1)

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  • Human CD11b/Integrin alpha M Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Neutrophils
    Species: Human
    Verified Customer | Posted 12/13/2021
    Human CD11b/Integrin alpha M Antibody (238439) MAB16992

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Protocols

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