Human CD11b/Integrin alpha M Antibody

Catalog # Availability Size / Price Qty
MAB16992
MAB16992-SP
Detection of CD11b/Integrin alpha M in Human Spleen.
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Product Details
Citations (9)
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Reviews (1)

Human CD11b/Integrin alpha M Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD11b/Integrin alpha M in direct ELISAs and Western blots. In Western blots no cross-reactivity with recombinant mouse (rm) Integrins alpha 2, alpha 3, alpha 4, alpha E or rhIntegrins alpha 5, alpha V, beta 1, beta 2, beta 3, or beta 6.
Source
Monoclonal Mouse IgG1 Clone # 238439
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alpha M
Phe17-Asn1105
Accession # NP_001139280
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Integrin  alpha M/CD11b
Immunohistochemistry
3-25 µg/mL
Human Spleen Tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of CD11b/Integrin alpha M in Human Spleen. CD11b/Integrin alpha M was detected in formalin fixed paraffin embedded sections of human spleen using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16992) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with Hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

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Citations for Human CD11b/Integrin alpha M Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. An integrated gene-to-outcome multimodal database for metabolic dysfunction-associated steatotic liver disease
    Authors: Kendall, TJ;Jimenez-Ramos, M;Turner, F;Ramachandran, P;Minnier, J;McColgan, MD;Alam, M;Ellis, H;Dunbar, DR;Kohnen, G;Konanahalli, P;Oien, KA;Bandiera, L;Menolascina, F;Juncker-Jensen, A;Alexander, D;Mayor, C;Guha, IN;Fallowfield, JA;
    Nature medicine
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. The Effect of Heterozygous Mutation of Adenylate Kinase 2 Gene on Neutrophil Differentiation
    Authors: T Horiguchi, A Tanimura, K Miyoshi, H Hagita, H Minami, T Noma
    International Journal of Molecular Sciences, 2022-12-17;23(24):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Synergism between obesity and HFpEF on neutrophils phenotype and its regulation by adipose tissue-molecules and SGLT2i dapagliflozin
    Authors: C Almengló, X Fu, MT Flores-Ari, ÁL Fernández, JE Viñuela, JM Martínez-C, D Durán, M Rodríguez-, JR González-J, S Eiras
    Journal of Cellular and Molecular Medicine, 2022-07-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in Atherosclerosis
    Authors: Jonathan Noonan, Gianluca Grassia, Neil MacRitchie, Paul Garside, Tomasz J. Guzik, Angela C. Bradshaw et al.
    Frontiers in Immunology
  5. Differential expression of adenine nucleotide converting enzymes in mitochondrial intermembrane space: a potential role of adenylate kinase isozyme 2 in neutrophil differentiation.
    Authors: Tanimura, Ayako, Horiguchi, Taigo, Miyoshi, Keiko, Hagita, Hiroko, Noma, Takafumi
    PLoS ONE, 2014-02-25;9(2):e89916.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. 1,25-Dihydroxyvitamin D3 promotes a sustained LPS-induced NF-kappaB-dependent expression of CD55 in human monocytic THP-1 cells.
    Authors: Izban M, Nowicki B, Nowicki S
    PLoS ONE, 2012-11-12;7(11):e49318.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
    BMC Neurosci, 2012-05-23;13(0):50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms.
    Authors: Nakayama H, Yoshizaki F, Prinetti A, Sonnino S, Mauri L, Takamori K, Ogawa H, Iwabuchi K
    J. Leukoc. Biol., 2007-11-30;83(3):728-41.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Protein-tyrosine kinase Syk is required for pathogen engulfment in complement-mediated phagocytosis.
    Authors: Shi Y, Tohyama Y, Kadono T, He J, Shahjahan Miah SM, Hazama R, Tanaka C, Tohyama K, Yamamura H
    Blood, 2006-01-31;107(11):4554-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human CD11b/Integrin alpha M Antibody
By Anonymous on 12/13/2021
Application: WB Sample Tested: Neutrophils Species: Human