Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 299813
View all CD2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD2
Lys25-Asp209
Accession # P06729

Specificity

Detects human CD2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse CD2 or recombinant human CD2F-10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD2 Antibody

Detection of Human CD2 antibody by Western Blot.

Detection of Human CD2 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), MOLT-4 human acute lymphoblastic leukemia cell line, and HeLa human cervical epithelial carcinoma cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB1856) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD2 at approximately 48 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human CD2 antibody by Simple WesternTM.

Detection of Human CD2 by Simple WesternTM.

Simple Western lane view shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), and HeLa human cervical epithelial carcinoma cell line (negative control), loaded at 0.2 mg/mL. A specific band was detected for CD2 at approximately 74 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB1856). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of CD2 in human tonsil.

CD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB1856) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CD2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Simple Western

50 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line

Reviewed Applications

Read 1 review rated 5 using MAB1856 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD2

CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T cells, NK cells, B cells and some antigen presenting cells. Human CD2 binds human CD58 on antigen-presenting cells to induce co-stimulating signals in T cells.

Alternate Names

CD2, LFA-2, T11

Entrez Gene IDs

914 (Human); 12481 (Mouse); 102120798 (Cynomolgus Monkey)

Gene Symbol

CD2

UniProt

P06729

Additional CD2 Products

Product Documents for Human CD2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD2 Antibody

For research use only

Customer Reviews for Human CD2 Antibody (1)

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  • Human CD2 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human CD2 Antibody MAB1856

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Protocols

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