Human CD2 Antibody

Catalog # Availability Size / Price Qty
AF1856
AF1856-SP
Detection  of Human CD2 by Western Blot.
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Human CD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD2
Lys25-Asp209
Accession # P06729
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
Human whole blood lymphocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human CD2 by Western Blot. View Larger

Detection of Human CD2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), MOLT‑4 human acute lymphoblastic leukemia cell line, and HeLa human cervical epithelial carcinoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1856) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD2 at approximately 48 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection  of Human CD2 by Simple WesternTM. View Larger

Detection of Human CD2 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), and HeLa human cervical epithelial carcinoma cell line (negative control), loaded at 0.2 mg/mL. A specific band was detected for CD2 at approximately 77-87 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1856) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD2

CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T cells, NK cells, B cells and some antigen presenting cells. Human CD2 binds human CD58 on antigen-presenting cells to induce co-stimulating signals in T cells.

Entrez Gene IDs
914 (Human); 12481 (Mouse); 102120798 (Cynomolgus Monkey)
Alternate Names
CD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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