Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 4% cross-reactivity with recombinant human (rh) IL-2 R beta is observed and no cross-reactivity with rhIL-2 R gamma, rhIL-1 RI, rhIL-1 RII, rhIL-4 R, rhIL-5 R alpha, rhIL-6 R, rhIL‑7 R, rhIL-9 R, and rhIL-10 R is observed.
Monoclonal Mouse IgG1 Clone # 22722
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213 Accession # P01589
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A) under non-reducing conditions only
Immersion fixed frozen sections of human lymph node
Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 0.5-1.0 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CD25/IL‑2 R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB223) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody.
Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB223). The ND50 is typically 0.5‑1.0 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2 R alpha
IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.
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