Human CD30 Ligand/TNFSF8 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human and mouse CD30 Ligand in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) CD27 Ligand is observed and less than 1% cross-reactivity with rhCD40 Ligand is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD30 Ligand
    Gln63-Asp234
    Accession # P32971
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human CD30 Ligand/TNFSF8 (Catalog # 1028-CL) and Recombinant Mouse CD30 Ligand/TNFSF8 (Catalog # 732-CL)
  • Flow Cytometry
    0.25 µg/106 cells
    Human peripheral blood mononuclear cells activated with PMA and Ca2+ ionomycin
  • Immunohistochemistry
    0.1-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CD30 Ligand/TNFSF8 in Human Thymus. CD30 Ligand/TNFSF8 was detected in immersion fixed paraffin-embedded sections of human thymus using Goat Anti-Human CD30 Ligand/TNFSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1028) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Hassall's corpuscles. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Immunohistochemistry
CD30 Ligand/TNFSF8 in Human Placenta. CD30 Ligand/TNFSF8 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human CD30 Ligand/TNFSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1028) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in villi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD30 Ligand/TNFSF8

CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells. The specific receptor for CD30L is CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using the monoclonal antibody Ki-1. CD30 is also expressed on different non-Hodgkin's lymphomas, virus-infected T and B cells, and on normal T and B cells after activation. Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and IL-4 receptor. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. CD30 can act as a costimulatory molecule in thymic negative selection and may also play a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. Human and mouse CD30 ligand cDNAs share 70% sequence homology.

  • References:
    1. Brunangelo, F. et al. (1995) Blood 85:1.
    2. Gruss, H-J. and F. Herrmann (1996) Leukemia and Lymphoma 20:397.
    3. Chiarle, R. et al. (1999) J. Immunol. 163:194.
  • Entrez Gene IDs:
    944 (Human); 21949 (Mouse)
  • Alternate Names:
    CD153 antigen; CD153; CD30 antigen ligand; CD30 Ligand; CD30L; CD30-L; CD30LCD30 ligand; CD30LGMGC138144; TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand superfamily member 8
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Selective redox regulation of cytokine receptor signaling by extracellular thioredoxin-1.
    Authors: Schwertassek U, Balmer Y, Gutscher M, Weingarten L, Preuss M, Engelhard J, Winkler M, Dick TP
    EMBO J., 2007;26(13):3086-97.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. CD40 induces macrophage anti-Toxoplasma gondii activity by triggering autophagy-dependent fusion of pathogen-containing vacuoles and lysosomes.
    Authors: Andrade RM, Wessendarp M, Gubbels MJ, Striepen B, Subauste CS
    J. Clin. Invest., 2006;116(9):2366-77.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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