Human CDX2 Antibody

Catalog # Availability Size / Price Qty
MAB3665-100
MAB3665-SP
CDX2 in  SW480 Human Cell Line.
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Human CDX2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CDX2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 963809
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CDX2
Asn54-Thr185
Accession # Q99626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry CDX2 in  SW480 Human Cell Line. View Larger

CDX2 in SW480 Human Cell Line. CDX2 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Mouse Anti-Human CDX2 Monoclonal Antibody (Catalog # MAB3665) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry CDX2 in  Human Colon Cancer Tissue. View Larger

CDX2 in Human Colon Cancer Tissue. CDX2 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human CDX2 Monoclonal Antibody (Catalog # MAB3665) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDX2

Human CDX2 (Caudal type homeobox transcription factor 2) is a 37 kDa member of the caudal family of homeobox proteins. It is 313 amino acids (aa) in length and contains a 167 aa caudal-like activation domain (aa 13‑180), a DNA-binding homeodomain (aa 186‑245) and two short, poly-glutamine and -proline repeats. CDX2 can exist as a dimer and is regulated by phosphorylation of a 4(x)serine motif that promotes CDX2 degradation. CDX2 promotes intestinal patterning and development. While it is necessary but not sufficient for intestinal phenotype differentiation, it will block cell proliferation on its own. Over the amino acid range used for immunization, human CDX2 is 95% and 97% aa identical to mouse and canine CDX2, respectively.

Long Name
Caudal-type Homeobox Transcription Factor 2
Entrez Gene IDs
1045 (Human); 12591 (Mouse); 66019 (Rat)
Alternate Names
caudal type homeo box transcription factor 2; caudal type homeobox 2; Caudal-type homeobox protein 2; CDX2; CDX3; CDX-3; CDX3caudal type homeobox transcription factor 2; homeobox protein CDX-2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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