Human Chorionic Gonadotropin alpha Chain (HCG alpha) Antibody (381012)

R&D Systems | Catalog # MAB4169

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 381012
View all Chorionic Gonadotropin alpha Chain (HCG alpha) Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chorionic Gonadotropin, alpha Chain ( alpha HCG)
Ala25-Ser116
Accession # P01215

Specificity

Detects human Chorionic Gonadotropin, alpha Chain ( alpha HCG) in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human FSH  beta or recombinant rat FSH  beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images

Chorionic Gonadotropin, a Chain (a HCG) antibody in Human Placenta by Immunohistochemistry (IHC-P).

Chorionic Gonadotropin, alpha Chain ( alpha HCG) in Human Placenta.

Chorionic Gonadotropin, a Chain (a HCG) was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Chorionic Gonadotropin, a Chain (a HCG) Monoclonal Antibody (Catalog # MAB4169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: Recombinant Human Chorionic Gonadotropin, alpha  Chain ( alpha  HCG)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB4169 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chorionic Gonadotropin alpha Chain (HCG alpha)

Human Chorionic Gonadotropin, alpha chain (CGA) is a 92 aa glycopeptide that functions as the shared alpha subunit of the heterodimeric peptide hormones choriogonadotropin, leutinizing hormone, thryoid stimulating hormone, and follicle stimulating hormone. CGA circulates as a free molecule and in noncovalent complexes with the unique beta subunits of those hormones. CGA is secreted by the pituitary and placenta. Mature human CGA shares 69%‑73% aa sequence identity with canine, equine, feline, mouse, porcine, and rat CGA.

Alternate Names

alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA

Entrez Gene IDs

1081 (Human)

Gene Symbol

CGA

UniProt

P01215

Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices

For research use only

Citations for Human Chorionic Gonadotropin alpha Chain (HCG alpha) Antibody (381012)

Customer Reviews (2)

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  • Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Placental tissue
    Species: Human
    Verified Customer | Posted 05/03/2022
    incubation 15 µg/mL overnight at 4 °C
    Human Chorionic Gonadotropin alpha Chain (HCG alpha) Antibody (381012) MAB4169
  • Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: embryonic stem cells and induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 12/15/2016
    We use the monoclonal antibody to detect human Chorionic Gonadotropin, alpha (CGA) Chain expressed in human embryonic stem cells and induced pluripotent stem cells (PSC) derived model for studying human placental trophoblasts in vitro. This antibody has been used with immunofluorescent methods to identify precursors of syncytiotrophoblasts emerging from PSC differentiation. The trophoblasts generated from human PSC most likely represents the primitive placenta encountered in early pregnancy soon after the human trophoblasts invades into the uterine wall.

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Protocols

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