Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Chorionic Gonadotropin, alpha Chain ( alpha HCG) in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human FSH  beta or recombinant rat FSH  beta is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 381012
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Chorionic Gonadotropin, alpha Chain ( alpha HCG)
    Ala25-Ser116
    Accession # P01215
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Chorionic Gonadotropin, alpha  Chain ( alpha  HCG)
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Chorionic Gonadotropin, alpha  Chain ( alpha  HCG) in Human Placenta. Chorionic Gonadotropin, alpha  Chain ( alpha  HCG) was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Chorionic Gonadotropin, alpha  Chain ( alpha  HCG) Monoclonal Antibody (Catalog # MAB4169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chorionic Gonadotropin alpha Chain (HCG alpha)

Human Chorionic Gonadotropin, alpha chain (CGA) is a 92 aa glycopeptide that functions as the shared alpha subunit of the heterodimeric peptide hormones choriogonadotropin, leutinizing hormone, thryoid stimulating hormone, and follicle stimulating hormone. CGA circulates as a free molecule and in noncovalent complexes with the unique beta subunits of those hormones. CGA is secreted by the pituitary and placenta. Mature human CGA shares 69%‑73% aa sequence identity with canine, equine, feline, mouse, porcine, and rat CGA.

  • Entrez Gene IDs:
    1081 (Human)
  • Alternate Names:
    alpha HCG; CGA glycoprotein hormones, alpha polypeptide; CGA; CG-ALPHA; Chorionic Gonadotropin alpha Chain; FSHA; GPHa; GPHa1; HCG; LHA; TSHA
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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  Very Good
 ICC/IF Human Anonymous 12/15/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedembryonic stem cells,induced pluripotent stem cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsWe use the monoclonal antibody to detect human Chorionic Gonadotropin, alpha (CGA) Chain expressed in human embryonic stem cells and induced pluripotent stem cells (PSC) derived model for studying human placental trophoblasts in vitro. This antibody has been used with immunofluorescent methods to identify precursors of syncytiotrophoblasts emerging from PSC differentiation. The trophoblasts generated from human PSC most likely represents the primitive placenta encountered in early pregnancy soon after the human trophoblasts invades into the uterine wall.

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