Detects human Chorionic Gonadotropin, alpha Chain ( alpha HCG) in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human FSH beta or recombinant rat FSH beta is observed.
Monoclonal Mouse IgG2A Clone # 381012
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Chorionic Gonadotropin, alpha Chain ( alpha HCG) Ala25-Ser116 Accession # P01215
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Chorionic Gonadotropin, alpha Chain ( alpha HCG) in Human Placenta.
Chorionic Gonadotropin, alpha Chain ( alpha HCG) was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Chorionic Gonadotropin, alpha Chain ( alpha HCG) Monoclonal Antibody (Catalog # MAB4169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Chorionic Gonadotropin, alpha chain (CGA) is a 92 aa glycopeptide that functions as the shared alpha subunit of the heterodimeric peptide hormones choriogonadotropin, leutinizing hormone, thryoid stimulating hormone, and follicle stimulating hormone. CGA circulates as a free molecule and in noncovalent complexes with the unique beta subunits of those hormones. CGA is secreted by the pituitary and placenta. Mature human CGA shares 69%‑73% aa sequence identity with canine, equine, feline, mouse, porcine, and rat CGA.
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We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.
We use the monoclonal antibody to detect human Chorionic Gonadotropin, alpha (CGA) Chain expressed in human embryonic stem cells and induced pluripotent stem cells (PSC) derived model for studying human placental trophoblasts in vitro. This antibody has been used with immunofluorescent methods to identify precursors of syncytiotrophoblasts emerging from PSC differentiation. The trophoblasts generated from human PSC most likely represents the primitive placenta encountered in early pregnancy soon after the human trophoblasts invades into the uterine wall.