Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody Summary
Accession # P01215
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chorionic Gonadotropin, alpha Chain ( alpha HCG) in Human Placenta. Chorionic Gonadotropin, a Chain (a HCG) was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Chorionic Gonadotropin, a Chain (a HCG) Monoclonal Antibody (Catalog # MAB4169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chorionic Gonadotropin alpha Chain (HCG alpha)
Human Chorionic Gonadotropin, alpha chain (CGA) is a 92 aa glycopeptide that functions as the shared alpha subunit of the heterodimeric peptide hormones choriogonadotropin, leutinizing hormone, thryoid stimulating hormone, and follicle stimulating hormone. CGA circulates as a free molecule and in noncovalent complexes with the unique beta subunits of those hormones. CGA is secreted by the pituitary and placenta. Mature human CGA shares 69%‑73% aa sequence identity with canine, equine, feline, mouse, porcine, and rat CGA.
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incubation 15 µg/mL overnight at 4 °C
We use the monoclonal antibody to detect human Chorionic Gonadotropin, alpha (CGA) Chain expressed in human embryonic stem cells and induced pluripotent stem cells (PSC) derived model for studying human placental trophoblasts in vitro. This antibody has been used with immunofluorescent methods to identify precursors of syncytiotrophoblasts emerging from PSC differentiation. The trophoblasts generated from human PSC most likely represents the primitive placenta encountered in early pregnancy soon after the human trophoblasts invades into the uterine wall.