Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 121723
View all CNTFR alpha Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha
Gln23-Pro346 (predicted)
Accession # P26992

Specificity

Detects human CNTF R alpha in direct ELISAs and Western blots. In Western blots, approximately 100% cross-reactivity with recombinant rat CNTF R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CNTFR alpha Antibody

Detection of CNTFR alpha in Human Skeletal Muscle.

CNTFR alpha was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human CNTFR alpha Monoclonal Antibody (Catalog # MAB303) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma and sarcoplasm of skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CNTFR alpha Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Skeletal Muscle

Western Blot

1 µg/mL
Sample: Recombinant Human CNTF R alpha (Catalog # 303-CR)

Reviewed Applications

Read 1 review rated 5 using MAB303 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CNTFR alpha

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha  subunit (CNTF R alpha ), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa  precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha  (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha.

Long Name

Ciliary Neurotrophic Factor Receptor alpha

Alternate Names

CNTF R alpha, CNTFR, CNTFRa

Entrez Gene IDs

1271 (Human); 12804 (Mouse); 313173 (Rat)

Gene Symbol

CNTFR

UniProt

P26992

Additional CNTFR alpha Products

Product Documents for Human CNTFR alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CNTFR alpha Antibody

For research use only

Related Research Areas

Diabetes
IL-6 Family

Citations for Human CNTFR alpha Antibody

Customer Reviews for Human CNTFR alpha Antibody (1)

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  • Human CNTFR alpha Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate tissue
    Species: Human
    Verified Customer | Posted 01/21/2022
    Human CNTFR alpha Antibody MAB303

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