Human Collybistin/ARHGEF9 Antibody

  (1 citations)     
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Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Detects human Collybistin/ARHGEF9 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 809309
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Collybistin/ARHGEF9
    Asn390-Lys516
    Accession # O43307
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Collybistin/ARHGEF9 by Western Blot. Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, human brain (cortex) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Collybistin/ARHGEF9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Collybistin/ARHGEF9 in Human Brain. Collybistin/ARHGEF9 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collybistin/ARHGEF9
Collybistin, also known as ARHGEF9, is an approximately 60 kDa cytoplasmic guanine nucleotide exchange factor. It is expressed in the brain and is upregulated at the onset of neuronal differentiation. Collybistin associates with Neuroligin-2, Gephyrin, and GABA receptor subunits to form a complex which is required for GABAergic synaptogenesis as well as hippocampal synaptic plasticity. Mutations in Collybistin are associated with a variety of neurological disorders including epilepsy, anxiety, aggression, mental retardation, and hyperplexia. Human Collybistin contains an SH3 domain (aa 8-67), a DH domain (aa 103-287), and a Pleckstrin homology domain (aa 318-426). Within aa 390-516, human Collybistin shares 97% and 57% aa sequence identity with mouse and rat Collybistin, respectively.
  • Long Name:
    Cdc42 Guanine Nucleotide Exchange Factor (GEF) 9
  • Entrez Gene IDs:
    23229 (Human); 236915 (Mouse); 66013 (Rat)
  • Alternate Names:
    ARHGEF9; Cdc42 guanine nucleotide exchange factor (GEF) 9; Collybistin; EIEE8; hPEM-2 collybistin; KIAA0424; KIAA0424HPEM-2; PEM-2 homolog; PEM2; PEM-2; Rac/Cdc42 guanine nucleotide exchange factor 9; rho guanine nucleotide exchange factor 9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Presynaptic neuronal pentraxin receptor organizes excitatory and inhibitory synapses
    Authors: Sung-Jin Lee
    J. Neurosci, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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