Human Collybistin/ARHGEF9 Antibody Summary
Accession # O43307
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse Collybistin/ARHGEF9 by Western Blot. Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, human brain (cortex) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Collybistin/ARHGEF9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Collybistin/ARHGEF9 in Human Brain. Collybistin/ARHGEF9 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Collybistin, also known as ARHGEF9, is an approximately 60 kDa cytoplasmic guanine nucleotide exchange factor. It is expressed in the brain and is upregulated at the onset of neuronal differentiation. Collybistin associates with Neuroligin-2, Gephyrin, and GABA receptor subunits to form a complex which is required for GABAergic synaptogenesis as well as hippocampal synaptic plasticity. Mutations in Collybistin are associated with a variety of neurological disorders including epilepsy, anxiety, aggression, mental retardation, and hyperplexia. Human Collybistin contains an SH3 domain (aa 8-67), a DH domain (aa 103-287), and a Pleckstrin homology domain (aa 318-426). Within aa 390-516, human Collybistin shares 97% and 57% aa sequence identity with mouse and rat Collybistin, respectively.
Citation for Human Collybistin/ARHGEF9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Presynaptic neuronal pentraxin receptor organizes excitatory and inhibitory synapses
Authors: Sung-Jin Lee
J. Neurosci, 2016;0(0):.
Sample Types: Whole Cells
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