Human Collybistin/ARHGEF9 Antibody

Catalog # Availability Size / Price Qty
MAB7848
MAB7848-SP
Detection of Human and Mouse Collybistin/ARHGEF9 by Western Blot.
2 Images
Product Details
Citations (1)
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Human Collybistin/ARHGEF9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Collybistin/ARHGEF9 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 809309
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Collybistin/ARHGEF9
Asn390-Lys516
Accession # O43307
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse Collybistin/ARHGEF9 by Western Blot. View Larger

Detection of Human and Mouse Collybistin/ARHGEF9 by Western Blot. Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, human brain (cortex) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Collybistin/ARHGEF9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Collybistin/ARHGEF9 in Human Brain. View Larger

Collybistin/ARHGEF9 in Human Brain. Collybistin/ARHGEF9 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collybistin/ARHGEF9

Collybistin, also known as ARHGEF9, is an approximately 60 kDa cytoplasmic guanine nucleotide exchange factor. It is expressed in the brain and is upregulated at the onset of neuronal differentiation. Collybistin associates with Neuroligin-2, Gephyrin, and GABA receptor subunits to form a complex which is required for GABAergic synaptogenesis as well as hippocampal synaptic plasticity. Mutations in Collybistin are associated with a variety of neurological disorders including epilepsy, anxiety, aggression, mental retardation, and hyperplexia. Human Collybistin contains an SH3 domain (aa 8-67), a DH domain (aa 103-287), and a Pleckstrin homology domain (aa 318-426). Within aa 390-516, human Collybistin shares 97% and 57% aa sequence identity with mouse and rat Collybistin, respectively.

Long Name
Cdc42 Guanine Nucleotide Exchange Factor (GEF) 9
Entrez Gene IDs
23229 (Human); 236915 (Mouse); 66013 (Rat)
Alternate Names
ARHGEF9; Cdc42 guanine nucleotide exchange factor (GEF) 9; Collybistin; EIEE8; hPEM-2 collybistin; KIAA0424; KIAA0424HPEM-2; PEM-2 homolog; PEM2; PEM-2; Rac/Cdc42 guanine nucleotide exchange factor 9; rho guanine nucleotide exchange factor 9

Product Datasheets

Citation for Human Collybistin/ARHGEF9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Presynaptic neuronal pentraxin receptor organizes excitatory and inhibitory synapses
    Authors: Sung-Jin Lee
    J. Neurosci, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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