Human CTRP5/C1qTNF5 Antibody

Catalog # Availability Size / Price Qty
MAB3167
MAB3167-SP
CTRP5/C1qTNF5 in Human Liver.
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Product Details
Citations (1)
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Human CTRP5/C1qTNF5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CTRP5/C1qTNF5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CTRP1 or rhCTRP4 is observed.
Source
Monoclonal Mouse IgG2B Clone # 332923
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CTRP5/C1qTNF5
Ser16-Ala243 (Gln44Arg)
Accession # Q9BXJ0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CTRP5/C1qTNF5
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry CTRP5/C1qTNF5 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

CTRP5/C1qTNF5 in Human Liver. CTRP5/C1qTNF5 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CTRP5/C1qTNF5 Monoclonal Antibody (Catalog # MAB3167) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of CTRP5/C1qTNF5 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CTRP5/C1qTNF5 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human CTRP5/C1qTNF5 Monoclonal Antibody (Catalog # MAB3167, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP5/C1qTNF5

CTRP5, also known as C1qTNF5, belongs to the highly conserved family of Acrp30/Adiponectin paralogs known as C1q and TNF‑related protein family. All family members share a modular organization comprising an N‑terminal signal peptide, a short variable region, a collagenous domain and a C‑terminal globular domain. CTRP proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. Human and mouse CTRP5 share 94% amino acid sequence identity.

Long Name
C1q And Tumor Necrosis Factor Related Protein 5
Entrez Gene IDs
114902 (Human); 235312 (Mouse)
Alternate Names
C1q and tumor necrosis factor related protein 5; C1qTNF5; Complement C1q Tumor Necrosis Factor-Related Protein 5 Precursor Variant 3; CTRP5; CTRP5LORDDKFZp586B0621; LORD 6; Myonectin 3

Product Datasheets

Citation for Human CTRP5/C1qTNF5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model
    Authors: E Melo, P Oertle, C Trepp, H Meisterman, T Burgoyne, L Sborgi, AC Cabrera, CY Chen, JC Hoflack, T Kam-Thong, R Schmucki, L Badi, N Flint, ZE Ghiani, F Delobel, C Stucki, G Gromo, A Einhaus, B Hornsperge, S Golling, J Siebourg-P, F Gerber, B Bohrmann, C Futter, T Dunkley, S Hiller, O Schilling, V Enzmann, S Fauser, M Plodinec, R Iacone
    EBioMedicine, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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