Human CXCL4/PF4 Antibody

Catalog #: MAB7952
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7952
MAB7952-SP

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Detection of Human CXCL4/PF4 by Western Blot.

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Citations (2)

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Human CXCL4/PF4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL4/PF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) CXCL6, recombinant human (rh) CXCL6, rhCXCL7, rmCXCL13 or rhCCL21 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 170138

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CXCL4/PF4
Glu32-Ser101
Accession # P02776.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

Human immature dentritic cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CXCL4/PF4 by Western Blot. Western blot shows lysates of human spleen tissue and human platelets. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7952) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL4/PF4

CXCL4, also known as Platelet Factor 4 (PF4), is an 8 kDa CXC chemokine that is stored in platelet alpha -granules as a homotetramer and secreted abundantly during platelet activation. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein that includes granule targeting and
heparin-binding sequences. CXCL4 has homology with IL-8 and beta -thromboglobulin and can form heteromultimers with IL-8. Mature human and mouse CXCL4 share 76% aa identity. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in
alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, has anti-angiogenic activity and promotes granule Protein C activation. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

References

Poncz, M. et al. (1987) Blood 69:219.
Scheuerer, B. et al. (2000) Blood 95:1158.
Perollet, C. et al. (1998) Blood 91:3289.
Petersen, F. et al. (1998) J. Immunol. 161:4347.
Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

Entrez Gene IDs

5196 (Human); 56744 (Mouse); 360918 (Rat)

Alternate Names

chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CXCL4/PF4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI.
Authors: Ollivier V, Syvannarath V, Gros A, Butt A, Loyau S, Jandrot-Perrus M, Ho-Tin-Noe B
PLoS ONE, 2014;9(8):e104712.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Functional divergence between 2 chemokines is conferred by single amino acid change.
Authors: Dubrac A, Quemener C, Lacazette E, Lopez F, Zanibellato C, Wu WG, Bikfalvi A, Prats H
Blood, 2010;116(22):4703-11.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay