Human CXCL4/PF4 Antibody Summary
Accession # P02776.2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CXCL4/PF4 by Western Blot. Western blot shows lysates of human spleen tissue and human platelets. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7952) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL4, also known as Platelet Factor 4 (PF4), is an 8 kDa CXC chemokine that is stored in platelet alpha -granules as a
homotetramer and secreted abundantly during platelet activation. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein that includes granule targeting and
heparin-binding sequences. CXCL4 has homology with IL-8 and beta -thromboglobulin and can form heteromultimers with IL-8. Mature human and mouse CXCL4 share 76% aa identity. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in
alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, has anti-angiogenic activity and promotes granule Protein C activation. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.
- Poncz, M. et al. (1987) Blood 69:219.
- Scheuerer, B. et al. (2000) Blood 95:1158.
- Perollet, C. et al. (1998) Blood 91:3289.
- Petersen, F. et al. (1998) J. Immunol. 161:4347.
- Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
- Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
Citations for Human CXCL4/PF4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI.
Authors: Ollivier V, Syvannarath V, Gros A, Butt A, Loyau S, Jandrot-Perrus M, Ho-Tin-Noe B
PLoS ONE, 2014;9(8):e104712.
Sample Types: Whole Cells
Applications: Flow Cytometry
Functional divergence between 2 chemokines is conferred by single amino acid change.
Authors: Dubrac A, Quemener C, Lacazette E, Lopez F, Zanibellato C, Wu WG, Bikfalvi A, Prats H
Sample Types: Recombinant Protein
Applications: Binding Assay
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human CXCL4/PF4 Antibody
There are currently no reviews for this product. Be the first to review Human CXCL4/PF4 Antibody and earn rewards!
Have you used Human CXCL4/PF4 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image