Human Cyclin E2 Antibody

Catalog # Availability Size / Price Qty
MAB7444
MAB7444-SP
Cyclin E2 in Human Breast.
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Human Cyclin E2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cyclin E2 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 718916
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Cyclin E2
Met1-Gln140
Accession # O96020
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Cyclin E2 in Human Breast. View Larger

Cyclin E2 in Human Breast.

Cyclin E2 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human Cyclin E2 Monoclonal Antibody (Catalog # MAB7444) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Cyclin E2 in Human Cervical Cancer Tissue. View Larger

Cyclin E2 in Human Cervical Cancer Tissue. Cyclin E2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human Cyclin E2 Monoclonal Antibody (Catalog # MAB7444) at 1.2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin E2

Cyclin E2, also known as CCNE2, is an approximately 45-50 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression and the initiation of DNA replication. Cyclin E2 is required for G1/S phase progression and cell cycle reentry from G0 phase. Cyclin E2 most highly expressed in testis, thymus, and brain and is frequently upregulated in cancer. Cyclin E2 contains two cyclin box folds (amino acids 141-231 and amino acids 241-361). An alternate splice form lacks 45 amino acids (aa) within the first cyclin box fold. Within aa 1-140, human Cyclin E2 shares 90% aa sequence identity with mouse and rat Cyclin E2.

Entrez Gene IDs
9134 (Human); 12448 (Mouse); 362485 (Rat)
Alternate Names
CCNE2; CYCE2; Cyclin E2; G1/S-specific cyclin E2; G1/S-specific cyclin-E2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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