DEK in HeLa Human Cell Line. DEK was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB70201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
DEK in Human Cervical Cancer Tissue. DEK was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB70201) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The DEK oncogene is an approximately 45 kDa phosphoprotein with pleiotropic activities. It is a chromatin remodeling factor, a transcriptional coactivator and corepressor, a component of RNA spliceosomes, and a promoter of DNA repair during apoptosis. DEK is upregulated in many cancers and promotes epithelial cell transformation. It functions as a proinflammatory factor when secreted by activated macrophages. It is an autoantigen in juvenile arthritis and lupus and a target of chromosomal translocation with the CAN gene in acute myelogenous leukemia. DEK contains one SAP domain (aa 149-183), one nuclear localization sequence (aa 205-221), and a DNA-binding C-terminal domain (aa 321-375). Within aa 292-375, human DEK shares 95% aa sequence identity with mouse and rat DEK.
Entrez Gene IDs:
7913 (Human); 110052 (Mouse); 306817 (Rat)
D6S231EDEK oncogene (DNA binding); DEK oncogene; protein DEK
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