Human DEK Antibody Summary
Accession # P35659
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human DEK by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and OVCAR-3 human ovarian carcinoma cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 30 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB7020) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEK at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DEK in Human Cervical Cancer Tissue. DEK was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB7020) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The DEK oncogene is an approximately 45 kDa phosphoprotein with pleiotropic activities. It is a chromatin remodeling factor, a transcriptional coactivator and corepressor, a component of RNA spliceosomes, and a promoter of DNA repair during apoptosis. DEK is upregulated in many cancers and promotes epithelial cell transformation. It functions as a proinflammatory factor when secreted by activated macrophages. It is an autoantigen in juvenile arthritis and lupus and a target of chromosomal translocation with the CAN gene in acute myelogenous leukemia. DEK contains one SAP domain (aa 149-183), one nuclear localization sequence (aa 205-221), and a DNA-binding C-terminal domain (aa 321-375). Within aa 292-375, human DEK shares 95% aa sequence identity with mouse and rat DEK.
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