Human DEK Antibody

Catalog # Availability Size / Price Qty
Detection of Human DEK by Western Blot.
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Product Details
Supplemental Products

Human DEK Antibody Summary

Species Reactivity
Detects human DEK in direct ELISAs and Western blots.
Monoclonal Mouse IgG2B Clone # 715524
Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell line HEK293EBNA-derived recombinant human DEK
Accession # P35659
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DEK antibody by Western Blot. View Larger

Detection of Human DEK by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and OVCAR-3 human ovarian carcinoma cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 30 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB7020) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEK at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry DEK antibody in Human Cervical Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

DEK in Human Cervical Cancer Tissue. DEK was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB7020) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEK

The DEK oncogene is an approximately 45 kDa phosphoprotein with pleiotropic activities. It is a chromatin remodeling factor, a transcriptional coactivator and corepressor, a component of RNA spliceosomes, and a promoter of DNA repair during apoptosis. DEK is upregulated in many cancers and promotes epithelial cell transformation. It functions as a proinflammatory factor when secreted by activated macrophages. It is an autoantigen in juvenile arthritis and lupus and a target of chromosomal translocation with the CAN gene in acute myelogenous leukemia. DEK contains one SAP domain (aa 149-183), one nuclear localization sequence (aa 205-221), and a DNA-binding C-terminal domain (aa 321-375). Within aa 292-375, human DEK shares 95% aa sequence identity with mouse and rat DEK.

Entrez Gene IDs
7913 (Human); 110052 (Mouse); 306817 (Rat)
Alternate Names
D6S231EDEK oncogene (DNA binding); DEK oncogene; DEK; protein DEK

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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