Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 129204
View all Desmoglein-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Desmoglein-1
Glu50-His545
Accession # Q02413

Specificity

Detects human Desmoglein-1 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Desmoglein-2 or rhDesmoglein-3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Desmoglein‑1 Antibody

Desmoglein-1 antibody in Human Skin Hyperplasia by Immunohistochemistry (IHC-P).

Desmoglein‑1 in Human Skin Hyperplasia.

Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocyte membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Desmoglein‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin hyperplasia

Western Blot

1 µg/mL
Sample: Recombinant Human Desmoglein‑1 Fc Chimera (Catalog # 944-DM)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB944 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Desmoglein-1

Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tail of Desmoglein-1 interacts with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the Desmoglein-1 with the intermediate filaments. Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters.

References

  1. Nollet, R. et al. (2000) J. Mol. Biol. 299:551.
  2. Elias, P. et al. (2001) J. Cell Biol. 153:243.

Alternate Names

CDHF4, Desmoglein1, DSG1

Entrez Gene IDs

1828 (Human)

Gene Symbol

DSG1

UniProt

Q02413

Additional Desmoglein-1 Products

Product Documents for Human Desmoglein‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Desmoglein‑1 Antibody

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Human Desmoglein‑1 Antibody

Customer Reviews for Human Desmoglein‑1 Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Human Desmoglein-1 Antibody
    Name: Agnès Tessier
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 02/27/2020
    Reconstructed human epidermis tissues were embedded in paraffin. Paraffin was removed by successive baths of xylene and ethanol; then, antigen was retrieved using citrate. A 1:100 dilution of the antibody was used (overnight incubation at 4°C).
    Human Desmoglein‑1 Antibody MAB944
  • Human Desmoglein-1 Antibody
    Name: Agnès Tessier
    Application: Simple Western
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 06/17/2019
    Demoglein expression in reconstructed human epidermis lysates (about 200 µg/mL protein) Antibody dilution 1:50, works also at 1:100 dilution
    Human Desmoglein‑1 Antibody MAB944

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Protocols

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