Human Doc2 alpha Antibody (842213)

R&D Systems | Catalog # MAB7904

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 842213
View all Doc2 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Doc2 alpha
Met1-Lys114
Accession # Q14183

Specificity

Detects human Doc2 alpha in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Doc2 beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Doc2 alpha Antibody (842213)

Doc2a antibody in Human Brain by Immunohistochemistry (IHC-P).

Doc2 alpha in Human Brain.

Doc2a was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human Doc2a Monoclonal Antibody (Catalog # MAB7904) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Doc2 alpha Antibody (842213)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Reviewed Applications

Read 1 review rated 5 using MAB7904 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Doc2 alpha

DOC2A/Doc2 alpha (Double C2-like domain containing protein alpha) is a 44 kDa (predicted) monomeric member of the C2 domain-containing protein family of molecules. It is expressed in both neurons and mast cells and appears to serve as an intracellular Ca++ sensor protein that regulates secretory vesicle release. In neurons, Doc2 alpha is normally bound to synaptic vesicles and interacts with Munc13-1 to promote secretory vesicle exocytosis through the cell membrane. In mast cells, a similar process occurs that involves Munc13-4 instead of Munc13-1. Human Doc2 alpha is 400 amino acids (aa) in length. It contains a Mid domain (aa 13-37) that binds Munc13‑1, followed by one C2 domain that binds Ca++ and lipid (aa 91-195) and a second C2 domain that binds SNAP25 (253-356). There is one potential alternative start site 16 aa upstream of the standard site. Over aa 1-114, human Doc2 alpha shares 90% aa sequence identity with mouse Doc2 alpha. Human DOC2B is the product of a separate gene and shares no meaningful aa sequence identity (<30%) with human Doc2 alpha.

Long Name

Double C2-like Domains, alpha

Alternate Names

DOC2A

Entrez Gene IDs

8448 (Human); 13446 (Mouse); 65031 (Rat)

Gene Symbol

DOC2A

UniProt

Q14183

Additional Doc2 alpha Products

Product Documents for Human Doc2 alpha Antibody (842213)

Certificate of Analysis

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Product Specific Notices for Human Doc2 alpha Antibody (842213)

For research use only

Customer Reviews for Human Doc2 alpha Antibody (842213) (1)

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  • Human Doc2 alpha Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 01/03/2022
    Human Doc2 alpha Antibody (842213) MAB7904

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