Human Doc2 alpha Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Doc2 alpha in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Doc2 beta is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 842213
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Doc2 alpha
    Met1-Lys114
    Accession # Q14183
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Doc2 alpha in Human Brain. Doc2 alpha was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human Doc2 alpha Monoclonal Antibody (Catalog # MAB7904) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Doc2 alpha
DOC2A/Doc2 alpha (Double C2-like domain containing protein alpha) is a 44 kDa (predicted) monomeric member of the C2 domain-containing protein family of molecules. It is expressed in both neurons and mast cells and appears to serve as an intracellular Ca++ sensor protein that regulates secretory vesicle release. In neurons, Doc2 alpha is normally bound to synaptic vesicles and interacts with Munc13-1 to promote secretory vesicle exocytosis through the cell membrane. In mast cells, a similar process occurs that involves Munc13-4 instead of Munc13-1. Human Doc2 alpha is 400 amino acids (aa) in length. It contains a Mid domain (aa 13-37) that binds Munc13‑1, followed by one C2 domain that binds Ca++ and lipid (aa 91-195) and a second C2 domain that binds SNAP25 (253-356). There is one potential alternative start site 16 aa upstream of the standard site. Over aa 1-114, human Doc2 alpha shares 90% aa sequence identity with mouse Doc2 alpha. Human DOC2B is the product of a separate gene and shares no meaningful aa sequence identity (<30%) with human Doc2 alpha.
  • Long Name:
    Double C2-like Domains, alpha
  • Entrez Gene IDs:
    8448 (Human); 13446 (Mouse); 65031 (Rat)
  • Alternate Names:
    Doc2 alpha; Doc2; DOC2A; doc2-alpha; double C2-like domain-containing protein alpha; double C2-like domains, alpha
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