Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 842213
View all Doc2 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Doc2 alpha
Met1-Lys114
Accession # Q14183

Specificity

Detects human Doc2 alpha in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Doc2 beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Doc2 alpha Antibody

Doc2a antibody in Human Brain by Immunohistochemistry (IHC-P).

Doc2 alpha in Human Brain.

Doc2a was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human Doc2a Monoclonal Antibody (Catalog # MAB7904) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Doc2 alpha Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Reviewed Applications

Read 1 review rated 5 using MAB7904 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Doc2 alpha

DOC2A/Doc2 alpha (Double C2-like domain containing protein alpha) is a 44 kDa (predicted) monomeric member of the C2 domain-containing protein family of molecules. It is expressed in both neurons and mast cells and appears to serve as an intracellular Ca++ sensor protein that regulates secretory vesicle release. In neurons, Doc2 alpha is normally bound to synaptic vesicles and interacts with Munc13-1 to promote secretory vesicle exocytosis through the cell membrane. In mast cells, a similar process occurs that involves Munc13-4 instead of Munc13-1. Human Doc2 alpha is 400 amino acids (aa) in length. It contains a Mid domain (aa 13-37) that binds Munc13‑1, followed by one C2 domain that binds Ca++ and lipid (aa 91-195) and a second C2 domain that binds SNAP25 (253-356). There is one potential alternative start site 16 aa upstream of the standard site. Over aa 1-114, human Doc2 alpha shares 90% aa sequence identity with mouse Doc2 alpha. Human DOC2B is the product of a separate gene and shares no meaningful aa sequence identity (<30%) with human Doc2 alpha.

Long Name

Double C2-like Domains, alpha

Alternate Names

DOC2A

Entrez Gene IDs

8448 (Human); 13446 (Mouse); 65031 (Rat)

Gene Symbol

DOC2A

UniProt

Q14183

Additional Doc2 alpha Products

Product Documents for Human Doc2 alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Doc2 alpha Antibody

For research use only

Customer Reviews for Human Doc2 alpha Antibody (1)

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  • Human Doc2 alpha Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 01/03/2022
    Human Doc2 alpha Antibody MAB7904

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