Human Doc2 alpha Antibody

Catalog # Availability Size / Price Qty
MAB7904
MAB7904-SP
Doc2 alpha  in Human Brain.
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Human Doc2 alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human Doc2 alpha in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Doc2 beta is observed.
Source
Monoclonal Mouse IgG2B Clone # 842213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Doc2 alpha
Met1-Lys114
Accession # Q14183
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Doc2a antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Doc2 alpha in Human Brain. Doc2a was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human Doc2a Monoclonal Antibody (Catalog # MAB7904) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Doc2 alpha

DOC2A/Doc2 alpha (Double C2-like domain containing protein alpha) is a 44 kDa (predicted) monomeric member of the C2 domain-containing protein family of molecules. It is expressed in both neurons and mast cells and appears to serve as an intracellular Ca++ sensor protein that regulates secretory vesicle release. In neurons, Doc2 alpha is normally bound to synaptic vesicles and interacts with Munc13-1 to promote secretory vesicle exocytosis through the cell membrane. In mast cells, a similar process occurs that involves Munc13-4 instead of Munc13-1. Human Doc2 alpha is 400 amino acids (aa) in length. It contains a Mid domain (aa 13-37) that binds Munc13‑1, followed by one C2 domain that binds Ca++ and lipid (aa 91-195) and a second C2 domain that binds SNAP25 (253-356). There is one potential alternative start site 16 aa upstream of the standard site. Over aa 1-114, human Doc2 alpha shares 90% aa sequence identity with mouse Doc2 alpha. Human DOC2B is the product of a separate gene and shares no meaningful aa sequence identity (<30%) with human Doc2 alpha.

Long Name
Double C2-like Domains, alpha
Entrez Gene IDs
8448 (Human); 13446 (Mouse); 65031 (Rat)
Alternate Names
Doc2 alpha; Doc2; DOC2A; doc2-alpha; double C2-like domain-containing protein alpha; double C2-like domains, alpha

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human Doc2 alpha Antibody
By Anonymous on 01/03/2022
Application: IHC Sample Tested: Brain tissue Species: Human