Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).
Human Dopa Decarboxylase/DDC Antibody (413911)
R&D Systems | Catalog # MAB3564
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 413911
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DDC
Met1-Glu480
Accession # P20711
Met1-Glu480
Accession # P20711
Specificity
Detects human DDC in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Dopa Decarboxylase/DDC Antibody (413911)
Detection of Dopa Decarboxylase/DDC Mouse by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Dopa Decarboxylase/DDC Specificity by Using Knockout Cell Line.
Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Dopa Decarboxylase/DDC Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Dopa Decarboxylase/DDC Antibody (413911)
Application
Recommended Usage
Knockout Validated
Dopa
Decarboxylase/DDC is specifically detected in Jurkat human acute T cell leukemia parental cell line but is
not detectable in Dopa Decarboxylase/DDC knockout Jurkat cell line.
Western Blot
2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Dopa Decarboxylase/DDC
References
- Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
- Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.
Alternate Names
AADC, DDC
Entrez Gene IDs
1644 (Human)
Gene Symbol
DDC
UniProt
Additional Dopa Decarboxylase/DDC Products
Product Documents for Human Dopa Decarboxylase/DDC Antibody (413911)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Dopa Decarboxylase/DDC Antibody (413911)
For research use only
Related Research Areas
Citations for Human Dopa Decarboxylase/DDC Antibody (413911)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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