Human Dopa Decarboxylase/DDC Antibody Summary
Accession # P20711
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Dopa Decarboxylase/DDC Mouse by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Dopa Decarboxylase/DDC Specificity by Using Knockout Cell Line. Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Dopa Decarboxylase/DDC Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dopa Decarboxylase/DDC
Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).
- Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
- Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.
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