Detection of Human Dynactin Subunit 2/DCTN2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Dynactin Subunit 2/DCTN2 Monoclonal Antibody (Catalog # MAB6850) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Dynactin Subunit 2/DCTN2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Dynactin Subunit 2/DCTN2 in HeLa Human Cell Line.
Dynactin Subunit 2/DCTN2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Dynactin Subunit 2/DCTN2 Monoclonal Antibody (Catalog # MAB6850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dynactin Subunit 2/DCTN2
DCTN2 (Dynactin-2; also 50 kDa Dynein-associated polypeptide and p50 Dynamitin) is a 50 kDa intracellular member of the Dynactin family of phosphoproteins. It is ubiquitously expressed and serves to stabilize the Dynactin complex of 11 different proteins. This complex is an obligate cofactor of Dynein and Kinesin motors, which position the mitotic spindle for cell division and move vesicles within the cell. Human Dynactin Subunit 2 is 401 amino acids (aa) in length. It contains three coiled‑coil regions (aa 99-132, 214-244 and 379-399), two potential phosphorylation sites (Ser83 and Tyr86), and one acetylation site at Ala2. The first two coiled‑coil regions contribute to oligomerization, while aa 1-91 are essential for Dynactin regulation. Potential isoform variants utilize either a five or two aa insertion after Ala35, an alternative start site at Met88, and a 21 aa substitution for aa 118-401. Over aa 277-401, human Dynactin Subunit 2 shares 95% aa identity with mouse Dynactin Subunit 2.
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