Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Control, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10825
View all EGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EGF

Specificity

Detects human EGF in direct ELISAs. Detects human EGF and rat EGF in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) HB‑EGF or rhTGF‑ alpha is observed. In Western blots, no cross-reactivity with recombinant mouse EGF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human EGF Antibody

Detection of Recombinant Human and Rat EGF antibody by Western Blot.

Detection of Recombinant Human and Rat EGF by Western Blot.

Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
EGF antibody in Human Skin by Immunohistochemistry (IHC-P).

EGF in Human Skin.

EGF was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody.

Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody.

Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND50 is typically 0.05-0.1 µg/mL.

Applications for Human EGF Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Western Blot

1 µg/mL
Sample: Recombinant Human EGF (Catalog # 236-EG)

Neutralization

Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.05-0.1 µg/mL in the presence of 2 ng/mL Recombinant Human EGF.

Reviewed Applications

Read 1 review rated 4 using MAB236 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGF

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Gene Symbol

EGF

Additional EGF Products

Product Documents for Human EGF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EGF Antibody

For research use only

Citations for Human EGF Antibody

Customer Reviews for Human EGF Antibody (1)

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  • Human EGF Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: DU145 human prostate carcinoma cell line
    Species: Human
    Verified Customer | Posted 11/12/2025
    Human EGF Antibody MAB236

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Protocols

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