Human EGF Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human EGF in direct ELISAs. Detects human EGF and rat EGF in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) HB‑EGF or rhTGF‑ alpha is observed. In Western blots, no cross-reactivity with recombinant mouse EGF is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 10825
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human EGF
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.05-0.1 µg/mL in the presence of 2 ng/mL Recombinant Human EGF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human and Rat EGF by Western Blot. Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
EGF in Human Skin. EGF was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND50 is typically
0.05‑0.1 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGF
EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.
  • Long Name:
    Epidermal Growth Factor
  • Entrez Gene IDs:
    1950 (Human); 13645 (Mouse); 25313 (Rat)
  • Alternate Names:
    beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Expression and clinical value of EGFR in human meningiomas
    Authors: MB Arnli, T Backer-Grø, B Ytterhus, US Granli, S Lydersen, S Gulati, SH Torp
    PeerJ, 2017;5(0):e3140.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells
    Authors: S Müller-Deu, L Seefried, M Krug, F Jakob, R Ebert
    Stem Cell Res, 2017;24(0):69-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  3. Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.
    Authors: Zhang Z, Dong Z, Lauxen I, Filho M, Nor J
    Cancer Res, 2014;74(10):2869-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
    Authors: Nadworny PL, Wang J, Tredget EE
    J Inflamm (Lond), 2010;7(0):13.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. S100A11, an dual mediator for growth regulation of human keratinocytes.
    Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
    Mol. Biol. Cell, 2007;19(1):78-85.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006;126(9):2096-105.
    Species: Human
    Sample Type: Serum
    Application: Neut
  7. Salivary leptin induces increased expression of growth factors in oral keratinocytes.
    Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
    J. Mol. Endocrinol., 2005;34(2):353-66.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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