Detects human EGF in direct ELISAs. Detects human EGF and rat EGF in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) HB‑EGF or rhTGF‑ alpha is observed. In Western blots, no cross-reactivity with recombinant mouse EGF is observed.
Monoclonal Mouse IgG1 Clone # 10825
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human EGF
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.05-0.1 µg/mL in the presence of 2 ng/mL Recombinant Human EGF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Recombinant Human and Rat EGF by Western Blot. Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
EGF in Human Skin. EGF was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND50 is typically 0.05‑0.1 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
EGF is the prototypic member of a family of growth factors that are
characterized by the presence of EGF like domains and activate members
of the EGF receptor family. Proteolytic cleavage of a membrane-bound
precursor releases mature soluble EGF which interacts with the EGF R to
promote proliferation and differentiation of mesenchymal and epithelial
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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