Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 494130
View all EphA10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EphA10
Glu34-Glu283 (predicted)
Accession # Q5JZY3

Specificity

Detects human EphA10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human EphA1, A2, A5, A6, B2, B3, B4, B6, recombinant mouse EphA3, A4, or A8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human EphA10 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Reviewed Applications

Read 2 reviews rated 5 using MAB5188 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: EphA10

EphA10 is a member of the Eph family of receptor tyrosine kinases that are activated by binding members of the Ephrin ligand family. EphA10 preferentially binds Ephrin-A3, -A4 and -A5.  EphA10 mRNA expression appears limited to the testis, unlike most Ephs that are expressed in developing and adult neural tissue. Human EphA10 extracellular domain (ECD) shares 95% and 88% amino acid identity with mouse and rat EphA10, respectively. Of three alternately spliced isoforms, one is truncated within the ECD and another within the cytoplasmic domain.

Long Name

Eph Receptor A10

Alternate Names

EC 2.7.10.1, EPH receptor A10, EphA10s protein, ephrin type-A receptor 10, FLJ16103, FLJ33655, MGC43817

Entrez Gene IDs

284656 (Human); 298528 (Rat)

Gene Symbol

EPHA10

UniProt

Q5JZY3 (Human)

Additional EphA10 Products

Product Documents for Human EphA10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human EphA10 Antibody

For research use only

Citations for Human EphA10 Antibody

Customer Reviews for Human EphA10 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human EphA10 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human EphA10 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human EphA10 Antibody MAB5188
  • Human EphA10 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 02/18/2019
    Human EphA10 Antibody MAB5188

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies