Human EphA10 Antibody

Name: Human EphA10 Antibody
Brand: R&D Systems
SKU: MAB5188-SP
Rating: 5 (1 reviews)

MAB5188 1 Review Datasheet / COA / SDS

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MAB5188
MAB5188-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EphA10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EphA10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human EphA1, A2, A5, A6, B2, B3, B4, B6, recombinant mouse EphA3, A4, or A8 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 494130

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human EphA10
Glu34-Glu283 (predicted)
Accession # Q5JZY3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA10

EphA10 is a member of the Eph family of receptor tyrosine kinases that are activated by binding members of the Ephrin ligand family. EphA10 preferentially binds Ephrin-A3, -A4 and -A5. EphA10 mRNA expression appears limited to the testis, unlike most Ephs that are expressed in developing and adult neural tissue. Human EphA10 extracellular domain (ECD) shares 95% and 88% amino acid identity with mouse and rat EphA10, respectively. Of three alternately spliced isoforms, one is truncated within the ECD and another within the cytoplasmic domain.

Long Name

Eph Receptor A10

Entrez Gene IDs

284656 (Human); 298528 (Rat)

Alternate Names

EC 2.7.10.1; EPH receptor A10; EphA10; EphA10s protein; ephrin type-A receptor 10; FLJ16103; FLJ33655; MGC43817