Human FABP5/E-FABP Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) FABP5 is observed and less than 5% cross-reactivity with recombinant human FABP1, -2, -3, -4, -6, -7, and rmFABP9 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human FABP5/E‑FABP
    Ala2-Glu135
    Accession # Q01469
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10-12.5 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FABP5/E‑FABP by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP5/E‑FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
FABP5/E‑FABP in HUVEC Human Umbilical Vein Endothelial Cells. FABP5/E‑FABP was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human FABP5/E‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E‑FABP at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Detection of Human FABP5/E‑FABP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E‑FABP at approximately 21 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP5/E-FABP
Human FABP-5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively.
  • Long Name:
    Fatty Acid-Binding Protein 5
  • Entrez Gene IDs:
    2171 (Human); 16592 (Mouse); 140868 (Rat)
  • Alternate Names:
    EFABP; E-FABP; E-FABPPAFABP; FABP5; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Curcumin restores sensitivity to retinoic acid in triple negative breast cancer cells.
    Authors: Thulasiraman P, McAndrews D, Mohiudddin I
    BMC Cancer, 2014;14(0):724.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Expression of fatty acid-binding proteins in adult hippocampal neurogenic niche of postischemic monkeys.
    Authors: Boneva NB, Kaplamadzhiev DB, Sahara S, Kikuchi H, Pyko IV, Kikuchi M, Tonchev AB, Yamashima T
    Hippocampus, 2011;21(2):162-71.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Type: Tissue Homogenates
    Application: WB
  3. Fatty acid-binding protein 5 and PPARbeta/delta are critical mediators of epidermal growth factor receptor-induced carcinoma cell growth.
    Authors: Kannan-Thulasiraman P, Seachrist DD, Mahabeleshwar GH, Jain MK, Noy N
    J. Biol. Chem., 2010;285(25):19106-15.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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