Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 121630
View all FGF-17 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-17
Thr23-Thr216
Accession # O60258

Specificity

Detects human FGF-17 in direct ELISAs and Western blots. In direct ELISA, approximately 7% cross-reactivity with recombinant human (rh) FGF-5 is observed and no cross-reactivity with rhFGF-acidic, rhFGF-basic, rhFGF-4, rhFGF-6, rhFGF-7, rhFGF8b, rhFGF-9, rhFGF-10, rhFGF-17, rhFGF-18, rhFGF-19, recombinant mouse (rm) FGF-8b, rmFGF-8c and rmFGF-15 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FGF‑17 Antibody

FGF‑17 antibody in Human Kidney by Immunohistochemistry (IHC-P).

FGF‑17 in Human Kidney.

FGF‑17 was detected in immersion fixed paraffin-embedded sections of human kidney using Human FGF‑17 Monoclonal Antibody (Catalog # MAB319) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the basement membrane supporting epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human FGF‑17 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

1 µg/mL
Sample: Recombinant Human FGF‑17 (Catalog # 319-FG)

Reviewed Applications

Read 1 review rated 5 using MAB319 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-17

Fibroblast growth factors (FGFs) play multiple biological functions including angiogenesis, mitogenesis, cellular differentiation and wound repairing. All members of the FGF family have a conserved approximately 120 amino acid core with 30‑70% identity. Among FGF family members, FGF-17 is most similar to FGF-8 (60% sequence identity) and FGF-18 (50% sequence identity). The mRNA of FGF-17 was found in midgestation of embryo and multiple adult tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. Human FGF‑17 shares 98.6% amino acid (aa) sequence identity with mouse FGF‑17. Rat FGF‑17 shares 100% aa sequence identity with mouse FGF‑17.

References

  1. Hoshikawa, M. et al. (1998) Biochem. Biophys. Res. Commun. 244:187.
  2. Xu, J. et al. (1999) Mech. Dev. 83:165.

Long Name

Fibroblast Growth Factor 17

Alternate Names

FGF17

Entrez Gene IDs

8822 (Human); 14171 (Mouse)

Gene Symbol

FGF17

UniProt

O60258

Additional FGF-17 Products

Product Documents for Human FGF‑17 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FGF‑17 Antibody

For research use only

Related Research Areas

FGF Family
FGF Ligands

Citations for Human FGF‑17 Antibody

Customer Reviews for Human FGF‑17 Antibody (1)

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  • Human FGF-17 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Kidney tissue
    Species: Human
    Verified Customer | Posted 06/28/2022
    Successful kidney staining
    Incubated kidney sample at 8 ug/mL overnight at 4C
    Human FGF‑17 Antibody MAB319

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Protocols

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