Detects human FGF-21 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse FGF-21, recombinant human (rh) FGF-3, -4, -6 or -10 is observed and no cross-reactivity with rhFGF-5 or rhFGF-9 is observed.
Monoclonal Mouse IgG2B Clone # 461804
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human FGF-21 His29-Ser209 Accession # Q9NSA1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of FGF‑21 in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25372, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
FGF‑21 in HepG2 Human Cell Line.
FGF‑21 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25372) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF-19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules (3). The cDNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region (4). Notably, FGF-21, as well as FGF-19 show limited binding to heparin (4). One potential alternate splice form has been reported. It shows a 43 aa substitution for the C‑terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6). The FGF‑19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction (7, 8, 9). FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex (3, 7, 10, 11). This situation occurs clinically during starvation, or following the ingestion of a high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation (11, 12).
Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
Huang, X. et al. (2006) Mol. Carcinog. 45:934.
Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
GenBank Accession #: EAW52401 (2006).
Ford, A.M. et al. (2005) J. Clin. Invest. 115:1627.
Moore, D. D. (2007) Science 316:1436.
Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.
Lundasen, T. et al. (2007) Biochem. Biophys. Res. Commun. 360:437.
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