Human FGF R3 (IIIc) Antibody MAB7662-100: R&D Systems

Human FGF R3 (IIIc) Antibody

Clone 136312 was used by HLDA to establish CD designation.
(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human FGF R3 (IIIc) in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with with recombinant human (rh) FGF R2 (IIIc), recombinant mouse (rm) FGF R2 (IIIc) and rmFGF R3 (IIIc) is observed. In Western blots (non-reducing conditions only), 50‑100% cross-reactivity with rhFGF R2 (IIIc), rmFGF R2 (IIIc) and rmFGF R3 (IIIc) is observed. Does not cross-react with the IIIb isoforms of rhFGF R3, rmFGF R3, rhFGF R2 or rmFGF R2. No cross-reactivity with any isoforms of rhFGF RI or rhFGF R4 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 136312
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human FGF R3 alpha (IIIc)
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR)
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human bladder cancer tissue
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
FGF R3 in U937 Human Cell Line. FGF R3 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human FGF R3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
FGF R3 in Human Bladder Cancer Tissue. FGF R3 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Mouse Anti-Human FGF R3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF R3
FGF R3 is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co-factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF-1, -2, -4, -8b, -8e, -8f, -9 and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.
  • Long Name:
    Fibroblast Growth Factor Receptor 3
  • Entrez Gene IDs:
    2261 (Human); 14184 (Mouse)
  • Alternate Names:
    CD333; CEK; EC 2.7.10; FGF R3; FGFR3; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Alternative splicing of fibroblast growth factor receptor 3 produces a secreted isoform that inhibits fibroblast growth factor-induced proliferation and is repressed in urothelial carcinoma cell lines.
    Authors: Tomlinson DC, L'Hôte CG, Kennedy W, Pitt E, Knowles MA
    Cancer Res., 2005;65(22):10441-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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