Detection of Human Fibronectin by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB19182) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fibronectin at approximately 260 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Fibronectin in HepG2 Human Cell Line.
Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with monensin using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB19182) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (punctate). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Fibronectin in Human Liver Cancer Tissue.
Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB19182) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in tumor cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibronectin is an extracellular matrix component that exists in
different alternately spliced isoforms. Fibronectin mediates cell
adhesion in its insoluble state but not as a soluble molecule.
Fibronectins play a role in cell adhesion, migration, differentiation,
and specific gene expression.
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