Human Flt-3 Ligand Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Flt‑3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Flt-3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt-3 Ligand
    Thr27-Pro185
    Accession # AAA17999
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Flt‑3 Ligand (Catalog # 308-FK)
  • Neutralization
    Measured by its ability to neutralize Flt‑3 Ligand-induced proliferation in BaFlt3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.02‑0.06 µg/mL in the presence of 5 ng/mL Recombinant Human Flt‑3 Ligand.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by Flt‑3 Ligand and Neutral­ization by Human Flt‑3 Ligand Antibody. Recombinant Human Flt‑3 Ligand (Catalog # 308-FK) stimulates proliferation in BaFlt3 mouse pro‑B cell line transfected with mouse Flt‑3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt‑3 Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Flt‑3 Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA). The ND50 is typically
0.02-0.06 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Flt-3 Ligand

Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1‑3). Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail (4‑7). Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity (4‑6). Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (4, 8). Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain (4, 5, 8). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 4, 6, 7). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 9). It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4‑6). It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation (1, 10). Animal studies also show Flt‑3 Ligand to reduce the severity of experimentally induced allergic inflammation (11).

  • References:
    1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
    2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
    3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
    4. Hannum, C. et al. (1994) Nature 368:643.
    5. Lyman, S.D. et al. (1994) Blood 83:2795.
    6. Lyman, S.D. et al. (1993) Cell 75:1157.
    7. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
    8. McClanahan, T. et al. (1996) Blood 88:3371.
    9. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
    10. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
    11. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.
  • Long Name:
    fms-like Tyrosine Kinase 3 Ligand
  • Entrez Gene IDs:
    2323 (Human); 14256 (Mouse); 493796 (Feline)
  • Alternate Names:
    FL; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Applications
Sample Type
  1. Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain.
    Authors: Perier A, Gourier C, Pichard S, Husson J, Lajeunesse E, Babon A, Menez A, Gillet D
    FEBS Lett., 2007;581(28):5480-4.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
    Authors: Solanilla A, Dechanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
    Blood, 2000;95(12):3758-64.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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